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Novel materials to enhance corneal epithelial cell migration on keratoprosthesis
  1. Akbar Karkhaneh1,
  2. Hamid Mirzadeh2,
  3. Alireza Ghaffariyeh3,
  4. Abdolali Ebrahimi4,
  5. Nazafarin Honarpisheh3,
  6. Masud Hosseinzadeh5,
  7. Mohammad Hossein Heidari4
  1. 1Islamic Azad University, Science and Research Branch, Faculty of Biomedical Engineering, Tehran, Iran
  2. 2Iran Polymer and Petrochemical Institute, Tehran, Iran
  3. 3Dr Khodadoust Eye Hospital, Shiraz, Iran
  4. 4Shahid Beheshti University, Tehran, Iran
  5. 5Fasa Medical School, Pathology Department, Fasa, Fars, Iran
  1. Correspondence to Dr Alireza Ghaffariyeh, Dr Khodadoust Eye Hospital, Motahari Avenue, Shiraz, Fars, Iran; alirezaghaffariyeh{at}


Purpose To introduce a new modification for silicone optical core Keratoprosthesis.

Methods Using mixtures of 2-hydroxyethyl methacrylate and acrylic acid polydimethylsiloxane (PDMS) films were modified with two-step oxygen plasma treatment, and then type I collagen was immobilised onto this modified surfaces. Both the biocompatibility of the modified films and cell behaviour on the surface of these films were investigated by in vitro tests, and formation of epithelial cell layer was evaluated by implantation of the modified films in the corneas of 10 rabbits.

Results In vitro studies indicated that the number of attached and proliferated cells onto modified PDMS in comparison with the unmodified PDMS significantly increased. Histological studies showed that corneal epithelial cells migrated on the anterior surface of the modified films after 1 week. The corneal epithelial cell formed an incomplete monolayer cellular sheet after 10 days. A complete epithelialisation on the modified surface was formed after 21 days. The epithelial layer persisted on the anterior surface of implant after 1-month and 3-month follow-up.

Conclusion This method may have potential use in silicone optical core Keratoprosthesis.

  • Keratoprosthesis
  • artificial cornea
  • surface modification
  • HEMA
  • acrylic acid
  • polydimethylsiloxane (PDMS)
  • collagen
  • ocular surface
  • cornea
  • experimental and laboratory
  • experimental and animal models
  • prosthesis

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  • See Editorial, p 304

  • Linked articles 188359.

  • Funding The authors express their appreciation to Fasa Medical School and Iran Polymer and Petrochemical Institute for financially supporting this work.

  • Competing interests None.

  • Patient consent Obtained.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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