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Laboratory science
Original article
Ocular surface molecule after transconjunctival vitrectomy
  1. Atsuko Fujita1,
  2. Eisuke Uchino1,
  3. Hiroki Otsuka1,
  4. Noboru Arimura1,
  5. Yoshihiro Noda2,
  6. Tatsuro Ishibashi2,
  7. Taiji Sakamoto1
  1. 1Department of Ophthalmology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
  2. 2Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
  1. Correspondence to Dr Taiji Sakamoto, Department of Ophthalmology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima 890-8520, Japan; tsakamot{at}m3.kufm.kagoshima-u.ac.jp

Abstract

Aim To evaluate ocular surface molecules after transconjunctival pars plana vitrectomy (PPV).

Methods A total of 28 eyes of 28 patients who received PPV were examined. Tears (10 μl) were collected before and after 20-gauge PPV or 23-gauge PPV. The concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and tumour necrosis factor (TNF)-α were measured. The concentrations of each cytokine before and after surgery were compared. The change ratios of each cytokine (post-/presurgery) between 20-gauge and 23-gauge PPV were compared.

Results IL-1β, IL-6, IL-8 increased after PPV (Wilcoxon rank sum test; IL-1 β, p=0.009; IL-6, p<0.001; IL-8, p<0.001), while the other cytokines did not. Among them, only IL-8 increased significantly in 20-gauge PPV than in 23-gauge PPV (p=0.01, Mann–Whitney U test). Multivariate analysis showed that 20-gauge PPV was a significant factor behind the increase in postoperative IL-8 (OR 5.21, 95% CI (1.46 to 18.55), p=0.018), but surgical time and simultaneous cataract surgery were not significant factors.

Conclusions The increase in inflammatory cytokine IL-8 in tear was significantly less after 23-gauge PPV than 20-gauge PPV, indicating that transconjunctival PPV is less invasive from the viewpoint of the ocular surface molecular biology. This information may be important when deciding the method of PPV.

  • Suture
  • 23 gauge
  • wound healing
  • irritation
  • tears
  • vitreous
  • retina

https://doi.org/10.1136/bjo.2010.185058

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    Footnotes

    • Funding This study was supported in part by Health and Labour Sciences Research Grants from the Ministry of Health, Labour and Welfare of Japan, Tokyo, Japan; the Ministry of Education, Culture, Sports, Science and Technology, Tokyo, Japan.

    • Competing interests None.

    • Patient consent Obtained.

    • Ethics approval Ethics approval was provided by the IRB of Kagoshima University Hospital, Kagoshima, Japan.

    • Provenance and peer review Not commissioned; externally peer reviewed.