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Toxoplasma gondii in the peripheral blood of patients with toxoplasmosis
Silveira et al studied 20 immunocompetent patients with acute, recurrent active, and with old toxoplasmic retinal scars. They also included 10 patients with circulating IgG antibodies against T gondii without ocular lesions. Blood samples were analysed by light microscopy, immunofluorescence assay, and confirmed by PCR amplification of parasite DNA. Tachyzoite-like organisms were found in the blood samples of 16 patients (9, light microscopy; 7, immunofluorescence assay). The results indicate that the parasite may circulate in the blood of immunocompetent individuals and that parasitaemia could be associated with the reactivation of the ocular disease. See page 396
Diagnosis of endophthalmitis by PCR
Sugita et al measured bacterial genome in 19 ocular fluid samples (8, aqueous; 11, vitreous) from 19 patients with suspected bacterial endophthalmitis, 50 ocular samples from uveitis patients, and 40 controls. Bacterial ribosomal DNA (16S rDNA) as measured by a quantitative PCR assay was detectable in 18 (95%) patients with suspected bacterial endophthalmitis, whereas only …
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