Article Text

Download PDFPDF
Characterisation of mouse limbal neurosphere cells: a potential cell source of functional neurons


Background/aims To characterise the origin, ultrastructure and functional properties of corneal limbal neurospheres (LNS).

Methods Limbal cells were isolated from the corneal limbus of adult mice and cultured in a serum-free sphere forming culture system. LNS were characterised by immunocytochemistry, Reverse-transcription-PCR and electron microscopy. LNS cells were also cocultured with neonatal mouse retinal cells. Phenotype and function were then assessed by immunofluorescence and a calcium influx/efflux assay.

Results LNS cells displayed clonal growth and self-renewal, and expressed a wide range of stem cell and neural lineage markers. The acquisition of neural properties was concordant with expression of neural crest markers including CD34, Sca1, Sox9, Twist1, but not CD45. LNS exhibited similar morphology and microstructure to neurospheres derived from the central nervous system. Following culture in a conducive environment, the derived cells displayed mature neural markers and exhibited electrical excitability.

Conclusions Corneal limbal stromal progenitor cells are a potential and convenient autologous cell source to generate functional neurons.

  • Experimental laboratory
  • Stem Cells
  • Cornea
  • Retina

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles

  • At a glance
    Harminder Singh Dua Arun D Singh