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Evaluation of three PCR assays for the detection of fungi in patients with mycotic keratitis
  1. Praveen Kumar Balne1,
  2. Ashok Kumar Reddy1,
  3. Manjulatha Kodiganti1,
  4. Sanyasi Rao Gorli1,
  5. Prashant Garg2
  1. 1Jhaveri Microbiology Centre, Prof Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L V Prasad Eye Institute, Hyderabad, India
  2. 2Cornea and Anterior Segment Services, L V Prasad Eye Institute, Hyderabad, India
  1. Correspondence to Dr Prashant Garg, L V Prasad Eye Institute, Banjara Hills, Road No. 2, Hyderabad 500 034, India; prashant{at}lvpei.org

https://doi.org/10.1136/bjophthalmol-2011-300207

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    Mycotic keratitis is a leading cause of ocular morbidity throughout the world and a higher incidence of fungal keratitis has been reported from tropical and subtropical countries. Early diagnosis is important for the prompt management of this disorder. Among the various diagnostic tests available, culture is considered the gold standard. The other commonly used techniques include direct microscopic examination methods like potassium hydroxide with or without calcofluor white wet mount, Grams stain, acridine orange and Giemsa stain.1 In the face of a negative culture, microscopy is relied upon for initiation of antifungal therapy.2 While the culture of fungus from clinical samples …

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    Footnotes

    • Funding Funding provided by the Hyderabad Eye Research Foundation, Hyderabad, India and Council of Scientific and Industrial Research, Government of India, New Delhi, India (grant 27(0191)/09/EMR-ІІ).

    • Competing interests None.

    • Patient consent Obtained.

    • Ethics approval Approval provided by the Institutional Review Board of L V Prasad Eye Institute.

    • Provenance and peer review Not commissioned; internally peer reviewed.