Article Text

Download PDFPDF
Blue light injures corneal epithelial cells in the mitotic phase in vitro
  1. Yoshimi Niwano1,
  2. Taro Kanno1,
  3. Atsuo Iwasawa2,
  4. Masahiko Ayaki3,
  5. Kazuo Tsubota3
  1. 1 Tohoku University Graduate School of Dentistry, Sendai, Japan
  2. 2 Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan
  3. 3 Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  1. Correspondence to Dr Masahiko Ayaki, Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan; mayaki{at}

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.


Blue light is a part of the visible light spectrum in the wavelength range of 390–490 nm. In modern society, light-emitting diode lamps and computer displays deliver much more blue light to the cornea than ever before. The retinal phototoxicity of blue light has been extensively investigated because of concerns about macular degeneration.1 ,2 The effects of blue light on the health of the ocular surface are less clear.3 ,4 Like skin,5 the ocular surface is directly exposed to visible blue light and this contributes to photophobia and ocular pain.6 We have carried out culture experiments to examine the phototoxicity of blue light in corneal cells.


A continuous-wave laser device equipped with an indium gallium nitride laser diode (RV-1000, Richo Optical Industry, Hanamaki, Japan) was used as a blue light source. Two rabbit corneal epithelial cell lines (SIRC and RC-1) were cultured in Dulbecco’s Modified Eagle Medium containing 10% fetal …

View Full Text


  • Correction notice This article has been corrected since it was published Online First. The initial sentence left over from the covering letter was removed.

  • Contributors YN and TK conducted and reported the study. AI, MA and KT were involved in the design and conduct of the study. YN and MA designed the study. MA and KT are the guarantors.

  • Competing interests None.

  • Provenance and peer review Not commissioned; internally peer reviewed.