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Amniotic membrane graft to conjunctival flap in treatment of non-viral resistant infectious keratitis: a randomised clinical study
  1. Bahaa-Eldin Hasan Abdulhalim1,
  2. Mostafa Mohamed Wagih1,
  3. Ahmed A M Gad1,
  4. Ghada Boghdadi2,
  5. Ragy R S Nagy3
  1. 1Department of Ophthalmology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  2. 2Department of Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  3. 3Department of Ophthalmology, El-Minia Eye Hospital, El-Minia, Egypt
  1. Correspondence to Professor Bahaa Hasan Abdulhalim, Department of Ophthalmology, Faculty of Medicine, Zagazig University, Zagazig, Egypt; bahaah2001{at}


Purpose To evaluate and compare the results of bipedicle conjunctival flap (CF) and cryopreserved amniotic membrane graft (AMG) in the treatment of non-viral infectious keratitis resistant to medical treatment.

Methods This prospective randomised interventional study included 40 eyes of 40 patients with resistant non-viral infectious keratitis. Twenty eyes received CF and 20 eyes received AMG. In the CF group, there were 12 eyes with fungal keratitis, 7 eyes with bacterial keratitis and 1 eye with Acanthamoeba keratitis. In the AMG group there were 13 eyes with fungal keratitis, 5 eyes with bacterial keratitis and 2 eyes with Acanthamoeba keratitis. In the CF group, three ulcers had descemetocele and four ulcers were perforated. In the AMG group, four ulcers had descemetocele and two ulcers were perforated. In CF, 360° peritomy was done and a bipedicle CF from the upper conjunctiva was dissected from Tenon's capsule, mobilised to cover the cornea and sutured to episclera. In AMG, one or two layers of AM were trimmed to fit the ulcer and sutured to the cornea. The follow-up period was 6 months.

Results Successful results were observed in 18/20 eyes (90%) in each group. Postoperatively, no significant differences between the two groups were found regarding success rate (p=1.0), epithelialisation time (p=0.75) or visual acuity improvement (p=0.84).

Conclusions CF and AMG are effective in treatment-resistant infectious keratitis. They could restore ocular surface integrity and provide metabolic and mechanical support for corneal healing. For large corneal perforation, it may be better to use another procedure such as penetrating keratoplasty to restore ocular integrity.

  • Cornea
  • Infection

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