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Human papillomavirus in normal conjunctival tissue and in conjunctival papilloma. Types and frequencies in a large series.
  1. Nicolai Christian Sjø,
  2. Christian von Buchwald,
  3. Patricia Cassonnet,
  4. Bodil Norrild,
  5. Jan Ulrik Prause,
  6. Troels Vinding,
  7. Steffen Heegaard (sh{at}
  1. Eye Pathology Institute, University of Copenhagen, Denmark
  2. Department of Oto-rhino-laryngology, Rigshospitalet, Copenhagen, Denmark
  3. Unite des papillomavirus, Institut Pasteur, Paris, France
  4. Protein Laboratory, University of Copenhagen, Denmark
  5. Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark
  6. Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark
  7. Eye Pathology Institute, University of Copenhagen, Denmark


    Aims: To examine conjunctival papilloma and normal conjunctival tissue for the presence of human papillomavirus (HPV).

    Methods: Archival paraffin embedded tissue from 165 conjunctival papillomas and from 20 histological normal conjunctival biopsies was analysed for the presence of HPV by polymerase chain reaction. Specimens considered HPV positive using consensus primers but with a negative or uncertain PCR result employing type specific HPV probes, were analysed with DNA sequencing.

    Results: HPV was present in 86 of 106 (81%) betaglobin positive papillomas. HPV type 6 was positive in 80 cases, HPV type 11 was identified in five cases and HPV type 45 was present in a single papilloma. All the 20 normal conjunctival biopsies were betaglobin positive and HPV negative.

    Conclusion: There is a strong association between HPV and conjunctival papilloma. Our study presents the largest material of conjunctival papilloma investigated for HPV and the first investigation of HPV in normal conjunctival tissue. HPV type 6 and 11 are the most common HPV types in conjunctival papilloma. This also is the first report of HPV type 45 in a conjunctival papilloma.

    • HPV
    • Papilloma
    • conjunctiva
    • papillomavirus

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