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Acanthamoeba keratitis therapy: time to cure and visual outcome analysis for different antiamoebic therapies in 227 cases
  1. Vincenzo Papa1,
  2. Paolo Rama2,
  3. Cherry Radford3,
  4. Darwin C Minassian4,
  5. John K G Dart5,6
  1. 1SIFI SpA, 36, Via Ercole Patti, Lavinaio, Italy
  2. 2Ophthalmol Cornea and Ocular Surface Unit, San Raffaele Scientific Institute, Milan, Italy
  3. 3Moorfields Eye Hospital NHS Foundation Trust, London, UK
  4. 4Epivision Ophthalmic Epidemiology Consultants, Penn, UK
  5. 5Corneal & External Diseaae, Moorfields Eye Hospital NHS Foundation Trust, London, UK
  6. 6Ocular Biology & Therapeutics, UCL Institute of Ophthalmology, London, UK
  1. Correspondence to Professor John K G Dart, Corneal & External Diseaae, Moorfields Eye Hospital NHS Foundation Trust, London EC1V 2PD, UK; J.Dart{at}ucl.ac.uk

Abstract

Aims To test the hypothesis that Acanthamoeba keratitis (AK) outcomes differ for different topical antiamoebic therapies (AAT) and to provide the detailed patient outcome data.

Methods A retrospective cohort study of 227 patients developing AK between 25 July 1991 and 10 August 2012. Inclusion criteria required a complete record of AAT treatment for both the primary outcome of a medical cure rate at 12 months and the secondary outcome of Snellen visual acuity ≤6/24 and/or surgical intervention. Analysis used multivariable regression to control for differences in baseline disease characteristics for both primary and secondary outcomes with unadjusted analyses for other outcomes. Subjects were categorised for analysis both by the AAT used at baseline and also by mutually exclusive AAT (patients exposed to all the drugs in each group, and no others, for some period). AAT categories were PHMB monotherapy, PHMB+diamidine, PHMB+chlorhexidine+diamidine, diamidine monotherapy and other AAT.

Results Analysis by baseline AAT showed no notable difference between treatments for both a medical cure at 12 months in 60.79% (138/227) or for a poor outcome in 49.34% (112/227). When AATs were analysed by mutually exclusive groups, PHMB monotherapy provided the best outcomes. These findings are subject to bias requiring careful interpretation. Overall cure rates for the 214 subjects with resolved outcomes were 94.27% (214/227), median time to cure 5 months (IQR 3.25–9.00 months) and range 1–26.24 months.

Conclusion PHMB 0.02% monotherapy for the initial treatment of AK is as effective as biguanide+diamidine combination therapy. Chlorhexidine monotherapy was too infrequent for comparison. The outcome data are the most detailed available.

  • cornea
  • drugs
  • infection
  • microbiology
  • treatment medical
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Footnotes

  • Contributors VP designed the study with input from DCM and JKGD; PR provided access to patients in Milan; CR reviewed the notes and prepared the data for analysis; DCM tabulated the data and carried out the statistical analysis; JKGD prepared the manuscript with input from all the other authors.

  • Funding SIFI S.p.A., 36, Via Ercole Patti, 95025 Lavinaio (Catania), Italy. Part of JKGD’s salary was paid by the National Institute of Research (NIHR) Biomedical Research Centre (BRC) at Moorfields Eye Hospital and the UCL Institute of Ophthalmology.

  • Competing interests VP is an employee of SIFI S.p.A. who manufacture and supply PHMB in Italy, and who are carrying out studies to develop it as a licensed therapy for the treatment of Acanthamoeba keratitis in Europe.

  • Patient consent for publication Not required.

  • Ethics approval The study was approved by the Moorfields Eye Hospital Clinical Research Management and Audit Department, and by the San Raffaele Hospital Ethics Committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as online supplementary information.

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