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Characterisation of human orbital fibroblasts cultivated from intraconal, nasal and central adipose tissues
  1. Jin A Kim1,
  2. Donghyuck Ahn1,
  3. Bo-Yeon Kim2,
  4. Yeon Jeong Choi1,
  5. Hyun Jung Shin1,
  6. Sun Young Jang1
  1. 1Department of Ophthalmology, Soonchunhyang University Bucheon Hospital, Bucheon, Gyeonggi-do, Republic of Korea
  2. 2Division of Endocrinology and Metabolism, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Republic of Korea
  1. Correspondence to Dr Sun Young Jang, Department of Ophthalmology, Soonchunhyang University Hospital Bucheon, Bucheon 14584, Republic of Korea; ysyat01{at}naver.com

Abstract

Purpose To investigate the characteristics of human orbital fibroblasts (OFs) cultivated from intraconal, nasal and central adipose tissues.

Methods Intraconal adipose tissues were obtained during orbital decompression surgery for severe proptosis in nine patients with Graves’ orbitopathy (GO). Nasal and central adipose tissues were obtained during upper eyelid blepharoplasty in nine patients with no history of GO. Human OFs were separately cultured from GO intraconal, non-GO nasal, non-GO central orbital adipose deposits. Human dermal fibroblasts were also cultured from redundant resected skin tissue obtained during upper eyelid blepharoplasty in normal controls. Expression of insulin-like growth factor 1 (IGF-1) and thyroid-stimulating hormone (TSH) receptors were investigated using real-time quantitative reverse transcription PCR. Protein levels of interleukin-1β (IL-1β)-induced inflammatory cytokines and generated intracellular reactive oxygen species (ROS) were determined.

Results IGF-1 and TSH receptor RNA expressions of GO intraconal OFs and non-GO nasal OFs were higher than non-GO central OFs and dermal fibroblasts. The expression of IL-1β induced the IL-6, IL-8, intercellular adhesion molecule-1 and cyclooxygenase-2 of GO intraconal OFs, and non-GO nasal OFs were higher than non-GO central OFs and dermal fibroblasts. Intracellular ROS generation in GO intraconal OFs and non-GO nasal OFs were higher than in non-GO central OFs and dermal fibroblasts, although the differences were not statistically significant.

Conclusions Non-GO nasal OFs had similar characteristics to GO intraconal OFs. We recommend the use of nasal adipose tissue in order to culture OFs as a normal control involving in vitro experiments.

  • Graves’ orbitopathy
  • orbital adipose tissue
  • orbital fibroblast
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Footnotes

  • JAK and DA contributed equally.

  • Contributors JAK, DA and SYJ were responsible for the study conception and design, as well as the intellectual content of the paper. YJC and HJS performed experiments. BYK, DA and SYJ revised the article critically for intellectual content. All authors read and approved the final manuscript.

  • Funding This study was supported by a grant from the National Research Foundation of Korea (NRF-2017R1A1A1A05001051) and the Soonchunhyang University Research Fund.

  • Competing interests None declared.

  • Patient consent for publication Obtained.

  • Ethics approval This study was approved by the Institutional Review Board of the Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as supplementary information.

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