Article Text

other Versions

Download PDFPDF
The effect of human platelet lysate on corneal nerve regeneration
  1. Chin-Te Huang1,2,
  2. Hsiao-Sang Chu1,3,4,5,
  3. Kuo-Chi Hung1,6,
  4. Lily Wei Chen1,
  5. Mei-Yun Chen1,
  6. Fung-Rong Hu1,5,
  7. Wei-Li Chen1,3,5
  1. 1Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  2. 2Department of Ophthalmology, Chung Shan Medical University Hospital, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
  3. 3Advanced Ocular Surface and Corneal Nerve Regeneration Center, National Taiwan University Hospital, Taipei, Taiwan
  4. 4Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
  5. 5Department of Ophthalmology, National Taiwan University College of Medicine, Taipei, Taiwan
  6. 6Department of Ophthalmology, Sinying Hospital, Tainan, Taiwan
  1. Correspondence to Professor Wei-Li Chen, Department of Ophthalmology, National Taiwan University Hospital, Taipei 10002, Taiwan; chenweili{at}


Aim This study aimed to test whether human platelet lysate (HPL) has neurotrophic ability for corneal nerve regeneration.

Methods We measured the neurotrophic factors in human peripheral serum (HPS) and two commercially available HPLs, UltraGRO and PLTMax. In vitro, we compared the growth rates, neuronal differentiation and immunostaining of neuron markers in mouse neuroblastoma cell line (Neuro-2a) and primary culture of mouse trigeminal ganglion cells that were cultivated in different concentrations of fetal bovine serum, HPS and HPL. In vivo, we created corneal wounds on Sprague Dawley rats with a rotating burr and evaluated the effects of topical HPL on wound healing and corneal nerve regeneration by in vivo confocal microscopy and corneal aesthesiometry.

Results HPLs had significantly higher concentrations of various neurotrophic factors compared with HPS (p<0.05). In Neuro-2a cells, 3% HPL was better at promoting neuronal growth and differentiation compared with HPS at the same concentration. HPL was also found to have superior neurotrophic effects compared with HPS in primary cultures of mouse trigeminal ganglion cells. In vivo, HPL-treated eyes had better corneal epithelial wound healing rate, nerve regeneration length and corneal touch threshold compared with eyes treated with artificial tears (p<0.05).

Conclusion HPL has significantly higher concentrations of neurotrophic factors compared with HPS. It showed not only in vitro but also in vivo corneal neurotrophic abilities. Our results suggest that HPL may have a potential role in the treatment of diseases related to corneal nerve damage or degeneration.

  • platelet lysate
  • corneal nerve regeneration
  • neurotrophic keratopathy

Statistics from


  • C-TH and H-SC are joint first authors.

  • Contributors W-LC conceived and planned the experiments and obtained institutional review board approvals. M-YC contributed to sample preparation and carried out the experiments. K-CH and F-RH contributed to the interpretation of the results. C-TH, H-SC and LWC took the lead in writing the manuscript. All authors provided critical feedback and helped shape the research, analysis and manuscript.

  • Funding This study was supported, in part, by the Department of Medical Research and the Advanced Ocular Surface and Corneal Nerve Regeneration Center at National Taiwan University Hospital in Taipei, Taiwan.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The preparation of the human peripheral serum in this study was approved by the Institutional Review Board for Human Studies at the National Taiwan University Hospital (201510123RINB). The animal use protocol has been approved by the Institutional Animal Care and Use Committee of National Taiwan University College of Medicine and College of Public Health (IACUC-20180120), and followed the ARVO Statement for the Use of Animal in Ophthalmic and Vision Research.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as supplementary information.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles

  • At a glance
    Frank Larkin