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Management of limbal stem cell deficiency by amnion-assisted conjunctival epithelial redirection using vacuum-dried amniotic membrane and fibrin glue
  1. Harminder Singh Dua1,2,
  2. Darren Shu Jeng Ting1,2,
  3. Ahmed AlSaadi3,
  4. Dalia G Said1,2
  1. 1Academic Ophthalmology, School of Medicine, University of Nottingham, Nottingham, UK
  2. 2Department of Ophthalmology, Queen's Medical Centre, Nottingham, UK
  3. 3Department of Ophthalmology, Zayed Military Hospital, Abu Dhabi, UAE
  1. Correspondence to Professor Harminder Singh Dua, Ophthalmology, University of Nottingham, Nottingham, NG7 2UH, UK; harminder.dua{at}nottingham.ac.uk

Abstract

Purpose To study the outcome of a modified amnion-assisted conjunctival epithelial redirection (ACER) technique using vacuum-dried amnion (Omnigen) and fibrin glue for managing total limbal stem cell deficiency (LSCD).

Method A retrospective, interventional case series of all patients with total LSCD who underwent limbal stem cell transplant (LSCT) using the modified ACER procedure between 2016 and 2019. The outcome was defined as: (1) success: complete corneal re-epithelialisation without conjunctivalisation; (2) partial success: sub-total corneal re-epithelialisation with partial non-progressive conjunctivalisation sparing the visual axis and (3) failure: conjunctivalisation affecting the visual axis.

Results Ten patients (six men), with a mean age of 46.2±18.4 years, were included. The mean follow-up was 23.0±13.9 months. Causes of LSCD were chemical eye injury (30%), congenital aniridia-related keratopathy (30%), ocular surface malignancy (20%), Steven-Johnson syndrome (10%) and contact lens overuse (10%). 50% were bilateral. The time from diagnosis to ACER (for acquired causes) was 45.6±44.4 months. 80% of patients achieved a complete/partial success following ACER and 20% of patients required repeat LSCT. Auto-LSCT was associated with a significantly higher chance of success than allo-LSCT (p=0.048). The mean best-corrected-visual-acuity (logMAR) improved significantly from 1.76±0.64 preoperatively to 0.94±0.94 at final follow-up (p=0.009). Omnigen was available off-the-shelf stored at room temperature and its transparency enabled visualisation of the healing epithelium beneath.

Conclusion LSCT using the modified ACER serves as an effective ocular surface reconstruction technique in managing total LSCD and improving vision. Vacuum-dried amnion provides advantages of easy handling, transparency and storage at room temperature.

  • ocular surface
  • cornea
  • stem cells

Data availability statement

Data are available upon reasonable request. Individual patient data are stored in the hospital’s electronic system and can be retrieved on reasonable request.

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Data availability statement

Data are available upon reasonable request. Individual patient data are stored in the hospital’s electronic system and can be retrieved on reasonable request.

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Footnotes

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests HSD is consultant to Artic Vision, Electrospinning, Santen, Thea and has shares in Glaxosmithkline and NuVision biotherapies (manufacturers of Omnigen) and is joint patent holder for the vacuum drying method for preparing Omnigen). None of the other authors have any conflict of interest to declare. This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors. HSD has received an unconditional educational grant from Santen, which is not related to the content of this paper.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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