TY - JOUR T1 - MIB-1 and PC-10 immunostaining for the assessment of proliferative activity in primary acquired melanosis without and with atypia JF - British Journal of Ophthalmology JO - Br J Ophthalmol SP - 1316 LP - 1319 DO - 10.1136/bjo.82.11.1316 VL - 82 IS - 11 AU - Itay Chowers AU - Nelly Livni AU - Abraham Solomon AU - Gershom Zajicek AU - Joseph Frucht-Pery AU - Robert Folberg AU - Jacob Pe’er Y1 - 1998/11/01 UR - http://bjo.bmj.com/content/82/11/1316.abstract N2 - AIMS To compare the proliferative activity of intraepithelial melanocytes in primary acquired melanosis (PAM) without atypia and PAM with atypia by immunohistochemical staining for the Ki-67 antigen and the proliferating cell nuclear antigen (PCNA). METHODS Formalin fixed, paraffin embedded sections from 35 archival specimens of PAM without atypia (n=19) and with atypia (n=16) were studied by immunostaining with MIB-1 and PC-10 monoclonal antibodies that react with the Ki-67 antigen and PCNA respectively. The results were calculated as the mean number of positive cells per eyepiece grid. All specimens were evaluated by two masked observers, and the interobserver reproducibility was assessed. RESULTS The means of the positive cell count in PAM with atypia were significantly higher compared with PAM without atypia for both observers, in both the PC-10 and the MIB-1 stained sections. In a linear least square model that estimated the interobserver and between group variation, the difference of MIB-1 and PC-10 positive cell count between PAM without and with atypia remained highly significant. The difference between the observers was not significant. CONCLUSIONS Immunostaining with MIB-1 and PC-10 demonstrated that PAM with atypia has higher proliferative activity than PAM without atypia. This method was found to be reproducible between different observers. ER -