PT  - JOURNAL ARTICLE
AU  - Masakazu Yamada
AU  - Hiroshi Mochizuki
AU  - Yuko Kamata
AU  - Yu Nakamura
AU  - Yukihiko Mashima
TI  - Quantitative analysis of lipid deposits from Schnyder’s corneal dystrophy
AID  - 10.1136/bjo.82.4.444
DP  - 1998 Apr 01
TA  - British Journal of Ophthalmology
PG  - 444--447
VI  - 82
IP  - 4
4099  - http://bjo.bmj.com/content/82/4/444.short
4100  - http://bjo.bmj.com/content/82/4/444.full
SO  - Br J Ophthalmol1998 Apr 01; 82
AB  - AIM To report the quantitation of the lipid composition of a corneal button from a Japanese woman in her 60s with clinically and histopathologically proved Schnyder’s corneal dystrophy. METHODS Total lipids extracted from the corneal button of the patient were analysed by the method of thin layer chromatography flame ionisation detection. Two different solvent systems were used for neutral lipid analysis and phospholipid analysis. Results were compared with three age matched corneal buttons obtained from cadaveric eyes. RESULTS The lipids that accumulated in the cornea in Schnyder’s dystrophy consisted mainly of unesterified cholesterol and phospholipids. The analysis of phospholipids showed sphingomyelin to be the predominant phospholipid in the patient’s cornea. CONCLUSION Findings suggest that this disorder involves a disturbance of the metabolism of cholesterol and/or sphingomyelin metabolism that is limited to the cornea.