PT  - JOURNAL ARTICLE
AU  - C Cuello
AU  - D Wakefield
AU  - N Di Girolamo
TI  - Neutrophil accumulation correlates with type IV collagenase/gelatinase activity in endotoxin induced uveitis
AID  - 10.1136/bjo.86.3.290
DP  - 2002 Mar 01
TA  - British Journal of Ophthalmology
PG  - 290--295
VI  - 86
IP  - 3
4099  - http://bjo.bmj.com/content/86/3/290.short
4100  - http://bjo.bmj.com/content/86/3/290.full
SO  - Br J Ophthalmol2002 Mar 01; 86
AB  - Background/aim: Anterior uveitis is a common inflammatory ocular disease characterised by protein accumulation and leucocyte infiltration in the anterior chamber. The aim of this study was to determine the expression of gelatinases in the aqueous humour (AH) and uvea in an animal model of endotoxin induced uveitis (EIU). Methods: EIU was established in Lewis rats following an intraperitoneal injection of lipopolysaccharide (LPS). AH and ocular tissue were obtained from control animals and those with EIU over a 1 week time course and the samples analysed immunohistochemically and by gelatin zymography. Results: Matrix metalloproteinase (MMP) 2 and 9 levels were elevated in rat AH over a 1 week time course. MMP-2 and MMP-9 levels peaked at the time of maximum uveal inflammation, before returning to baseline levels as the inflammation subsided. MMP-9 was detected in the latent and functionally active form. Total protein extracted from inflamed rat uveal tissue displayed no significant gelatinolytic modulation throughout the time course of EIU. Anterior chamber neutrophils and ciliary body epithelial cells were the most abundant source of the gelatinases. Conclusion: This study has revealed a correlation between infiltrating neutrophils and the presence of elevated gelatinases in EIU. The results suggest that these proteolytically active enzymes may be important mediators of the inflammatory response and contribute to matrix remodelling observed in uveitis. Furthermore, the excess production of MMPs may be a mechanism by which leucocytes, such as neutrophils, gain access to uveal tissue and AH. Therapeutic strategies aimed at reducing MMP activity may be of some benefit in the treatment of uveitis.