RT Journal Article SR Electronic T1 Confocal microscopy of Aspergillus fumigatus keratitis JF British Journal of Ophthalmology JO Br J Ophthalmol FD BMJ Publishing Group Ltd. SP 409 OP 410 DO 10.1136/bjo.87.4.409 VO 87 IS 4 A1 A M Avunduk A1 R W Beuerman A1 E D Varnell A1 H E Kaufman YR 2003 UL http://bjo.bmj.com/content/87/4/409.abstract AB Aim: To use a confocal microscope to characterise the treated and untreated courses of fungal keratitis. Methods: In the first experiment, Aspergillus fumigatus stromal keratitis was produced in both eyes of seven New Zealand white rabbits. In the second experiment, keratitis was induced in right eyes of 20 rabbits. Group 1 rabbits were treated with topical fluconazole, group 2 rabbits received oral fluconazole, and group 3 rabbits were used as controls. The rabbits were examined with a slit lamp and confocal microscope 2, 6, 10, 14, and 20 days after inoculation. The corneal cultures were taken on days 2, 14, and 20 and biopsies were taken on days 2 and 22. Results: On days 14 and 22 confocal microscopy was more sensitive than culture technique in both treated and untreated animals, since not all cases of fungal keratitis can be cultured. Conclusion: This study indicates that confocal microscopy is a rapid and sensitive diagnostic tool for both the early diagnosis and non-invasive follow up of fungal keratitis