RT Journal Article
SR Electronic
T1 Reproducibility of the Heidelberg retinal flowmeter in determining low perfusion areas in peripapillary retina
JF British Journal of Ophthalmology
JO Br J Ophthalmol
FD BMJ Publishing Group Ltd.
SP 1266
OP 1269
DO 10.1136/bjo.2003.039099
VO 88
IS 10
A1 C P Jonescu-Cuypers
A1 A Harris
A1 R Wilson
A1 L Kagemann
A1 L V Mavroudis
A1 F Topouzis
A1 A L Coleman
YR 2004
UL http://bjo.bmj.com/content/88/10/1266.abstract
AB Aim: To evaluate the interobserver variability and retest reproducibility of confocal scanning laser Doppler flowmeter in measuring capillary perfusion of the peripapillary retina. Methods: Blood flow measurements were performed in one eye of 10 normal subjects by two investigators on two different days (visits). Five separate measurements of the peripapillary blood flow parameters were recorded by each observer at each visit. The Heidelberg retina flowmeter was used to record capillary perfusion in a 2560×640 μm area of the superotemporal peripapillary region and pixel by pixel analysis was done from an area adjacent to the optic disc, with a minimum of 1600 pixels. The percentage of pixels with less than 1 arbitrary unit of flow (no flow) and 10, 25, 50, 75, 90th percentiles of flow values was calculated. Interobserver measurements were compared by paired t test. Intraclass correlations (ICC) were used to determine the interobserver variability and retest reproducibility of the measurements. Intrasession coefficients of variations (CV) were also calculated. Results: There were no statistically significant differences between the two observers for all measurements and between visits for the percentage of pixels with no flow. ICC was 66% (range 57.09%–77.1%) for pixels with no flow. For the 10, 25, 50, 75, 90th percentiles of flow the ICC was 63.07% (53.91%–77.81%), 71.3% (64.23%–80.85%), 72.61% (66.02%–78.96%), 65.86% (58.53%–74.77%), and 60.05% (54.34%–70.06%), respectively. CV was 16.59%, 11.47%, 9.32%, 9.03%, 11.58%, and 16.05% for the percentage of no flow pixels and the 10, 25, 50, 75, 90th percentiles of flow, respectively. Conclusions: The Heidelberg retinal flowmeter allows reproducible measurements of all levels of capillary perfusion areas when pixel by pixel analysis is used.