RT Journal Article
SR Electronic
T1 The role of clusterin in retinal development and free radical damage
JF British Journal of Ophthalmology
JO Br J Ophthalmol
FD BMJ Publishing Group Ltd.
SP 1541
OP 1546
DO 10.1136/bjo.2007.115220
VO 91
IS 11
A1 Jeong Hun Kim
A1 Jin Hyoung Kim
A1 Young Suk Yu
A1 Bon-Hong Min
A1 Kyu-Won Kim
YR 2007
UL http://bjo.bmj.com/content/91/11/1541.abstract
AB Aim: To assess the role of clusterin in retinal vascular development and in free radical damage in vivo and in vitro.Methods: The expression of clusterin, von Willebrand factor (vWF), flk-1, heat shock protein 27 (Hsp27) and heat shock protein 70 (Hsp70) was examined in the retinas of developing mice and oxygen-induced retinopathy (OIR) mice by immunofluorescence staining and western blot analysis. Hydrogen peroxide (H2O2)-pretreated human retinal endothelial cells (HREC) and astrocytes were cultured in the presence or absence of exogenous clusterin, and then the cell viability was measured using the MTT assay and DAPI staining.Results: Clusterin was expressed mainly in the inner retina and co-localised with vWF, an endothelial cell marker. During the mouse developmental process, clusterin expression was decreased, which was similar to the expression of flk-1, vWF and Hsp27. Furthermore, in the OIR model, clusterin expression changed in a similar way to both vWF and Hsp27. Under hypoxic conditions, clusterin expression increased in HREC and astrocytes. In H2O2-pretreated HREC and astrocytes, clusterin protected against apoptotic cell death.Conclusions: These results suggest that clusterin is associated with protection from apoptotic retinal cell death in retinal development and in free radical damage.