RT Journal Article
SR Electronic
T1 Pars planitis is associated with an increased frequency of effector-memory CD57+ T cells
JF British Journal of Ophthalmology
JO Br J Ophthalmol
FD BMJ Publishing Group Ltd.
SP 1393
OP 1398
DO 10.1136/bjo.2007.116277
VO 91
IS 10
A1 Miguel Pedroza-Seres
A1 Marisela Linares
A1 Stephanie Voorduin
A1 Rojas-Ramos Enrique
A1 Ricardo Lascurain
A1 Yonathan Garfias
A1 Maria Carmen Jimenez-Martinez
YR 2007
UL http://bjo.bmj.com/content/91/10/1393.abstract
AB Aim: To evaluate the frequency, phenotype and the potential function of CD57+ T cell subsets in patients with pars planitis.Methods: CD4+CD57+ and CD8+CD57+ T cells were quantitated in peripheral blood from 15 patients with pars planitis and 15 healthy controls. To evaluate the phenotype and potential function of CD57+ T cell subsets CCR7, CD27, CD28, CD45RA, CD45RO, intracellular IFN-γ, IL-4, perforin and granzyme-A expression were assessed by flow cytometry.Results: CD57+ T cells subsets were increased in patients with pars planitis (p = 0.002). The majority of CD4+CD57+ T cells were CCR7−CD27−CD28−CD45RO+, while the most CD8+CD57+ T cells were CCR7−CD27−CD28−CD45RA+. The number of cells positive for intracellular IFN-γ and IL-4 was higher in the CD57+ T cell populations. A greater number of CD8+CD57+ T cells than CD8+CD57− T cells were positive to perforin (p = 0.006) and granzyme-A (p = 0.01).Conclusions: CD57+ T cells had a phenotype associated with peripheral memory (CCR7−CD27−CD28−). Cytokine production by CD57+ T cells suggests that these cells may play a role in helper cell regulation. High expression of intracellular proteins involved in cytotoxicity suggests that CD8+CD57+ T cells may play an effector role. Taken together, this study proposes that CD57+ T cells function as memory-effector T cell subsets during pars planitis pathogenesis.