TY - JOUR T1 - Floating device for donor corneas in organ culture JF - British Journal of Ophthalmology JO - Br J Ophthalmol SP - 1676 LP - 1678 DO - 10.1136/bjo.2008.140574 VL - 92 IS - 12 AU - J T Lie AU - F M Lock AU - P G H Mulder AU - J van der Wees AU - G R J Melles Y1 - 2008/12/01 UR - http://bjo.bmj.com/content/92/12/1676.abstract N2 - Aim: To introduce a new floating device for donor corneas to avoid accumulation of debris onto the endothelial surface during organ culture and to facilitate handling of the tissue during preservation and surgery. Methods: From 11 donors, one randomly chosen cornea was stored in organ culture attached to a floating device, while the contralateral cornea was attached to the lid of the phial by a suture (“hanging by suture”). Endothelial cell density (ECD) was evaluated prior to tissue storage and after 2–3 weeks of culture. Furthermore, we compared ECD in a larger group of corneas sent off for transplantation with the device (n = 281) to a historical group of control corneas “hanging by suture” (n = 444). Results: There was no significant difference in ECD between corneas attached to the floating device or “hanging by suture” (n = 11; p⩾0.1). Similarly, no different ECDs were observed between corneas sent off for transplantation with the device (n = 281) and the historical group of control corneas “hanging by suture” (n = 444) (p⩾0.1). Conclusion: The use of the floating device may not affect tissue quality. Since its introduction, the use of the device has been uneventful and greatly facilitated tissue handling. ER -