RT Journal Article
SR Electronic
T1 Aqueous humor levels of asymmetric dimethylarginine (ADMA) are positively associated with monocyte chemoattractant protein-1 (MCP-1) in patients with uveitis
JF British Journal of Ophthalmology
JO Br J Ophthalmol
FD BMJ Publishing Group Ltd.
SP 93
OP 94
DO 10.1136/bjo.2007.124966
VO 92
IS 1
A1 Y Yoshida
A1 S Yamagishi
A1 S Ueda
A1 K Yoshimura
A1 S Okuda
A1 R Yamakawa
YR 2008
UL http://bjo.bmj.com/content/92/1/93.abstract
AB Background/aims: The aim of the study was to evaluate whether aqueous humor levels of asymmetric dimethylarginine (ADMA) are associated with monocyte chemoattractant protein-1 (MCP-1).Methods: Aqueous humor levels of ADMA and MCP-1 were measured by high-performance liquid chromatography (HPLC) and ELISA, respectively, in 31 uveitis samples and nine cataract control samples.Results: Aqueous humor ADMA and MCP-1 levels were significantly higher in infectious or non-infectious uveitis patients than in controls (0.67±0.04 nmol/ml vs 0.55±0.03 nmol/ml vs 0.43±0. 04 nmol/ml (p<0.01) and 29.0±11.3 ng/ml vs 4.5±1.2 ng/ml vs 0.47±0.1 ng/ml (p<0.01), respectively). A positive correlation between ADMA and MCP-1 levels in aqueous humor was found in control and uveitis patients (r = 0.33, p<0.05).Conclusion: The results demonstrated that aqueous humor levels of ADMA were positively associated with MCP-1 in humans. Our present observations suggest that aqueous humor levels of ADMA may be a novel biomarker of inflammation in uveitis.