RT Journal Article SR Electronic T1 Microbial contamination of multiply used preservative-free artificial tears packed in reclosable containers JF British Journal of Ophthalmology JO Br J Ophthalmol FD BMJ Publishing Group Ltd. SP 1518 OP 1521 DO 10.1136/bjo.2008.144469 VO 92 IS 11 A1 Kim, M S A1 Choi, C Y A1 Kim, J M A1 Chung, H R A1 Woo, H Y YR 2008 UL http://bjo.bmj.com/content/92/11/1518.abstract AB Background/aims: To evaluate microbial contamination of multiply used preservative-free artificial tears packed in reclosable containers after daily use.Methods: Subjects were provided with preservative-free artificial tears (Groups 1 and 2) and artificial tears containing a preservative (Group 3). After administration three times or more per 10 h, bottles were collected, and any remaining fluid in the bottles was cultured. A risk factor analysis for microbial contamination was performed by the use of univariate and multivariate analysis.Results: A total of 242 eye-drop bottles were evaluated. Five (2.0%) of the 242 bottles had bacterial contamination. In group 1, four (3.9%) of 102 bottles were contaminated, and identified bacteria were all coagulase-negative Staphylococcus. In group 2, one (1.0%) of 105 bottles was contaminated, and it was a Gram-negative Acinetobacter species. No bottles from group 3 showed any contamination. Based on multivariate analysis, advanced age and fingertip touch were statistically significant risk factors for microbial contamination (p<0.05).Conclusion: Preservative-free artificial tears in reclosable containers are at risk of contamination in a daily and multiple use setting, especially in patients with a poor administering technique, which is associated with fingertip touch and advanced age.