TY - JOUR T1 - Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis JF - British Journal of Ophthalmology JO - Br J Ophthalmol SP - 1195 LP - 1200 DO - 10.1136/bjo.2007.136044 VL - 92 IS - 9 AU - R Duan AU - J M van Dun AU - L Remeijer AU - M Siemerink AU - P G H Mulder AU - P Norberg AU - A D M E Osterhaus AU - G M G M Verjans Y1 - 2008/09/01 UR - http://bjo.bmj.com/content/92/9/1195.abstract N2 - Aim: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).Methods: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.Results: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.Conclusions: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype. ER -