PT  - JOURNAL ARTICLE
AU  - H Yera
AU  - O Zamfir
AU  - T Bourcier
AU  - E Viscogliosi
AU  - C Noël
AU  - J Dupouy-Camet
AU  - C Chaumeil
TI  - The genotypic characterisation of <em>Acanthamoeba</em> isolates from human ocular samples
AID  - 10.1136/bjo.2007.132266
DP  - 2008 Aug 01
TA  - British Journal of Ophthalmology
PG  - 1139--1141
VI  - 92
IP  - 8
4099  - http://bjo.bmj.com/content/92/8/1139.short
4100  - http://bjo.bmj.com/content/92/8/1139.full
SO  - Br J Ophthalmol2008 Aug 01; 92
AB  - Background: We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis.Methods: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis.Results: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis.Conclusion: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.