@article {Amato519, author = {Domenico Amato and Francesco Oddone and Mario Nubile and Rossella Anna Maria Colabelli Gisoldi and Carlo Maria Villani and Augusto Pocobelli}, title = {Pre-cut donor tissue for Descemet stripping automated keratoplasty: anterior hinged lamella on versus off}, volume = {94}, number = {4}, pages = {519--522}, year = {2010}, doi = {10.1136/bjo.2009.161158}, publisher = {BMJ Publishing Group Ltd}, abstract = {Aims To compare two different techniques for preparation of pre-cut lamellar corneal tissue for Descemet stripping automated keratoplasty.Background/Methods Eight donor lenticules were created with a full pass of the microkeratome blade, which resulted in a posterior lamella and a free cap (anterior lamella off group (AL-off)). Contralateral donor lenticules were created with an incomplete pass of the microkeratome blade, which resulted in a posterior lamella and a hinged anterior cap (anterior lamella on group (AL-on)). Endothelial cell density, cellular viability and corneal thickness were evaluated before dissection, and 4 and 24 h after dissection.Results Average pre-cut endothelial cell density was 2552.25 ({\textpm}105) and 2572.25 ({\textpm}110) cells/mm2 in the AL-off group and AL-on group, respectively (p=0.7). At 24 h cell density was 2404.87 ({\textpm}74) in the AL-off group (p\<0.01) and 2368.74 ({\textpm}148) in the AL-on group (p=0.01). The mean percentage of trypan blue-stained cells was consistently higher in the hinged AL-on group. Corneal thickness increased by approximately 20\% in both groups after 24 h cold storage.Conclusion Eye bank-prepared tissue offers the potential advantage of being screened for mechanical stress damage occurring during the automated dissection.}, issn = {0007-1161}, URL = {https://bjo.bmj.com/content/94/4/519}, eprint = {https://bjo.bmj.com/content/94/4/519.full.pdf}, journal = {British Journal of Ophthalmology} }