PT  - JOURNAL ARTICLE
AU  - Ng, Zhi Xiang
AU  - Kuppusamy, Umah Rani
AU  - Tajunisah, Iqbal
AU  - Fong, Kenneth Choong Sian
AU  - Koay, Adrian Choon Aun
AU  - Chua, Kek Heng
TI  - 2245G/A polymorphism of the receptor for advanced glycation end-products (<em>RAGE</em>) gene is associated with diabetic retinopathy in the Malaysian population
AID  - 10.1136/bjophthalmol-2011-300658
DP  - 2012 Feb 01
TA  - British Journal of Ophthalmology
PG  - 289--292
VI  - 96
IP  - 2
4099  - http://bjo.bmj.com/content/96/2/289.short
4100  - http://bjo.bmj.com/content/96/2/289.full
SO  - Br J Ophthalmol2012 Feb 01; 96
AB  - Background/aims The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of diabetic microvascular complications. The aim of this study was to investigate the association between 2245G/A gene polymorphism of the RAGE gene and retinopathy in Malaysian type 2 diabetic patients.Methods 342 unrelated type 2 diabetic patients (171 with retinopathy (DR), 171 without retinopathy (DNR)) and 235 unrelated healthy subjects from all over Malaysia were recruited for this study. Genomic DNA was isolated from 3 ml samples of whole blood using a modified conventional DNA extraction method. The genotype and allele frequencies of 2245G/A were studied using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method.Results A statistically significant difference in 2245A minor allele frequency was found between control (5.5%) and DR groups (15.2%) (p<0.001, OR=3.06, 95% CI 1.87 to 5.02) as well as between DNR (8.2%) and DR (15.2%) groups (p<0.01, OR=2.01, 95% CI 1.24 to 3.27). However, when the frequency was compared between control and DNR groups, there was no significant difference (p>0.05).Conclusions This is the first study that shows an association between the 2245A allele of the RAGE gene and development of diabetic retinopathy in the Malaysian population.