Table 1

Monoclonal antibodies used in this study

Antibody (clone)SpecificityWorking dilutionSource
CD 49 DVLA-4 α chain1:20Dakopatts
CD 11aLFA-1 α chain1:20Dakopatts
CD 18LFA-1 β chain1:20Dakopatts
CD 54ICAM-11:20Dakopatts
CD 50ICAM-31:20Dakopatts
CD 106VCAM-11:10Dakopatts
CD 62 EE-selectin (ELAM-1)1:100Dakopatts
HLA-DRMHC class II antigens1:50Becton-Dickinson
OKT4T helper/inducer cells1:20Ortho Diagnostics
Leu 3aT helper/inducer cells1:5Becton-Dickinson
OKT8T cytotoxic/suppressor cells1:10Ortho Diagnostics
L26Pan B cells1:100Dakopatts
KP1Macrophages1:50Dakopatts
  • Location of manufacturers: Dakopatts A/S, Copenhagen, Denmark; Becton-Dickinson, Sunnyvale, CA, USA; Ortho Diagnostics, Raritan, NJ, USA.

  • VLA-4 = very late activation antigen-4; LFA-1 = lymphocyte function associated antigen-1; ICAM-1 = intercellular adhesion molecule-1; ICAM-3 = intercellular adhesion molecule-3, VCAM-1 = vascular cell adhesion molecule-1; ELAM-1 = endothelial leucocyte adhesion molecule-1; MHC = major histocompatibility complex.

  • OKT4 and Leu3a were applied simultaneously.