Table 2

Primer and probe sequences used in nested polymerase chain reaction (modified from Nawa et al10)

Sequence 5′ - 3′LocationProduct length
Outer ACCGAAAACGGTTGAACCGAAAACGGT AATAATGTCTATATTCACTAATT—307 bp
Inner:
 HPV 16ATGTTTCAGGACCCACAGGA104–123 124 bp
CCTCACGTCGCAGTAACTGT208–227
 HPV 18ATGGCGCGCTTTGAGGATCC106–125
GCATGCGGTATACTGTCTCT274–293188 bp
Probe:
 HPV 16CACAGTTATGCACAGAGCTGCAAACAAC
TATACATGAT		141–180—
 HPV 18GAACTGAACACTTCACTGCAAGACATAG
AAATAACCTG		151–190

  • ↵Consensus primers for E60RF of HPV 16 and 18.