Immunohistochemical detection of cell adhesion molecules (ICAM-1, E-selectin and CD44) and vascular endothelial growth factor (VEGF) in laser treated rats
| Tissue | Time post laser | ICAM -1 | E-selection | CD44 | VEGF | |
|---|---|---|---|---|---|---|
| Retinal layer | Untreated | ELM, ILM, GL | − | ELM, ILM, GL | − | |
| 3–5 days | ELM, ILM, GL | − | ELM, ILM, GL | − | ||
| 2 weeks | ELM, ILM, GL | − | ELM, ILM, GL | − | ||
| RPE cells | Untreated | − | − | − | + | |
| 3–5 days | + + | + | + + | + + + | ||
| 2 weeks | + | − | − | + | ||
| Choroidal layer | Untreated | − | − | − | + | |
| 3–5 days | + + + VEC, IF, | + VEC, IF | + + + VEC, IF | + + + VEC, IVL, IF | ||
| 2 weeks | − | − | ± | + | ||
ELM= external limiting membrane; ILM = internal limiting membrane; GL = ganglion cell layer; VEC = vascular endothelial cells; IF = infiltrating cells; IVL = intravascular leucocytes. − no signal; ± very weak signal, 1–3 cells/view; + weak signal, 4–6 cells/view; ++ medium signal, 7–10 cells/view; +++ strong signal, >10 cells/view.