Table 1

Absolute cell numbers (×10⁴)/animal (two retinas)

Cell populations/days post immunisation		Controls				Tolerised
Cell populations/days post immunisation		7	14			7	14
CD4⁺αβTCR⁺	1.62	109 (130)		1.44	69.7 (58.3)
CD8⁺αβTCR⁺	1.17	42 (47.1)		1.86	28.7 (16.8)
ED7⁺CD4⁺	24.3	67.1 (54.8)		12.3	24.6 (37.3)
ED7⁺MHC class II⁺	nd	109.8		nd	98.4
OX22⁺CD4⁺	nd	51		nd	41

↵Figures are a representative experiment and calculated from the mean recovery from two animals in each group (four retinas). (Cell numbers for a second experiment in parenthesis.)
↵Cells were backgated from CD45(OX1)⁺ positive cells (see Methods) and only ED7^high cells were included in analysis of macrophage infiltrate thereby excluding resident microglia (ED7^low expressing; see Dick et al 22) which did not change in number between groups in any of the experiments.
↵See Figure 3 and legend.
↵Athough MHC class II expression was increased on majority of CD45(OX1)⁺ cells greater than negative control, increased numbers of ED7⁺ cells expressed MHC class II in control retinas (see Fig 3).