Table 1

Identification of androgen receptor protein in epithelial cell nuclei of human ocular tissues and cells

TissueSexAgePathologyAndrogen receptor staining
1st Antibody without competitor1st Antibody with “AR 462-478” peptide1st Antibody with “AR 1-21” peptide
Lacrimal glandfemale70++
Lacrimal glandmaleelderlyhypertrophy++
Meibomian glandfemale48ectropion++
Meibomian glandfemale70entropion++
Meibomian glandmale85++
Meibomian glandmale88chalazion++
Bulbar conjunctivafemale77++
Bulbar conjunctivafemale79cataract++
Bulbar conjunctivamale27corneal scar and cataract++
Bulbar conjunctivamale38keratoconus++
Bulbar conjunctivamale70aphakia++
Bulbar conjunctivamale85cataract++
Forniceal conjunctivamale55++
Corneafemale65herpes simplex keratitis+
Corneamale27corneal scar and cataract++
Lens epithelial cellsmale13cataract++
RPE cellsmale71cardiac arrest++
  • RPE = retinal pigment epithelial.

  • Tissues and cells were obtained and processed as described in the Materials and methods. To verify the specificity of staining, aliquots of the rabbit anti-human androgen receptor antibody were preincubated with excess amounts of either peptide “AR 1-21”, which was used to elicit the original antibody, or peptide “AR 462-478”, which was derived from a non-reactive part of the androgen receptor. These aliquots, or the first antibody alone (that is, without a competiting peptide), were then used in the immunoperoxidase procedure. Under these conditions, the AR 1-21, but not the AR 462-478, peptide should prevent specific androgen receptor staining.