Tissue | Sex | Age | Pathology | 5α-Reductase mRNA | |||||
---|---|---|---|---|---|---|---|---|---|
Type 1 | Type 2 | ||||||||
Lacrimal gland | female | 82 | sebaceous gland carcinoma | + | + | ||||
Lacrimal gland | male | elderly | hypertrophy | + | + | ||||
Lacrimal gland | male | 80 | + | + | |||||
Meibomian gland | female | 82 | sebaceous gland carcinoma | + | + | ||||
Meibomian gland | female | 79 | ectropion | − | − | ||||
Meibomian gland | male | 58 | ectropion | + | + | ||||
Meibomian gland | male | 68 | ectropion | + | + | ||||
Meibomian gland | male | 74 | ectropion | + | + | ||||
Bulbar conjunctiva* | female | 30, 74, 75 | cataract, cataract, cataract | + | + | ||||
Bulbar conjunctiva* | male | 32, 66, 76 | pterygium, pterygium, cataract | + | + | ||||
Cornea† | female | 30, 33, 85 | HSK, HSK, corneal scar | + | + | ||||
Cornea† | male | 48, 73, 82 | HSK, transplant rejection, ABK | + | + | ||||
Cornea‡ | male | 83 | heart disease | + | + | ||||
RPE cells§ | male | 71 | cardiac arrest | + | + |
HSK = herpes simplex keratitis; ABK = aphakic bullous keratopathy.
Tissues and cells were obtained and processed either individually or as combined preparations for molecular biological analysis. The designated samples of bulbar conjunctiva* and cornea† were pools of three female or three male tissues. Similarly, two corneas‡ purchased from the eye bank were combined into one sample. The RPE (retinal pigment epithelial) cells originated from two separate cultures, which were derived from the same tissue. Both RPE cell preparations were positive for types 1 and 2 5α-reductase mRNA. Data are reported as the presence (+) or absence (−) of the specific reductase isozyme, as determined by RT-PCR and agarose gel electrophoresis.