Table 2

Presence of types 1 and 2 5α-reductase mRNA in human ocular tissues and cells

TissueSexAgePathology5α-Reductase mRNA
Type 1Type 2
Lacrimal glandfemale82sebaceous gland carcinoma++
Lacrimal glandmaleelderlyhypertrophy++
Lacrimal glandmale80++
Meibomian glandfemale82sebaceous gland carcinoma++
Meibomian glandfemale79ectropion
Meibomian glandmale58ectropion++
Meibomian glandmale68ectropion++
Meibomian glandmale74ectropion++
Bulbar conjunctiva* female30, 74, 75cataract, cataract, cataract++
Bulbar conjunctiva* male32, 66, 76pterygium, pterygium, cataract++
Cornea female30, 33, 85HSK, HSK, corneal scar++
Cornea male48, 73, 82HSK, transplant rejection, ABK++
Cornea male83heart disease++
RPE cells§ male71cardiac arrest++
  • HSK = herpes simplex keratitis; ABK = aphakic bullous keratopathy.

  • Tissues and cells were obtained and processed either individually or as combined preparations for molecular biological analysis. The designated samples of bulbar conjunctiva* and cornea were pools of three female or three male tissues. Similarly, two corneas purchased from the eye bank were combined into one sample. The RPE (retinal pigment epithelial) cells originated from two separate cultures, which were derived from the same tissue. Both RPE cell preparations were positive for types 1 and 2 5α-reductase mRNA. Data are reported as the presence (+) or absence (−) of the specific reductase isozyme, as determined by RT-PCR and agarose gel electrophoresis.