Table 1

Oligonucleotide primers used for analysing the GPR75 gene

Amplicon Primer name Sequence Size (bp) Restriction enzyme (sizes in bp) TA (°C)
191.R7 5′-TAATCCCTTTCTGTTTCTGG-3′ 348NlaIII (201+147) 53
91.S4 5′-AGGACAAGAAGACAATGAAG-3′
291.R1 5′-ATCTCATCCACACAGCCAC-3′ 318Mnl I (180+138) 59
91.S8 5′-CGATCACTGCCACTGTCTTC-3′
391.R2 5′-CCAGTTCAGGCTTCATCATC-3′ 439Acc I (257+182) 60
91.S7 5′-CCGGCATGGCACACTGGAT-3′
491.R3 5′-TGTGCAGGGAGGTGGAGAT-3′ 303Mnl I (172+131) 60
91.S6 5′-AAACTGGTAAAGAATGAAGC-3′
591.R4 5′-GTCCTGGTGTGCTGTCTTCC-3′ 327Hae III (196+131) 56
91.S2 5′-CTGTGGCTTTGGAGATAACA-3′
691.R5 5′-CAGAAACAAATCCTCCCATC-3′ 349Alu I (190+159) 60
91.S1 5′-TCTGCTTGGCTGAAGTGCTG-3′
791.R9 5′-AACTCTTTTGGATTTGCC-3′248- 54
91.S11 5′-GAAAGAAAACCATAACTGCC-3′
  • PCR conditions were 94°C, 5 minutes; 30 cycles of 94°C, 30 seconds, TA, 30 seconds, 72°C, 30 seconds; 72°C, 5 minutes.