PCR primer sequences
| Fragment | Forward primer (name, sequence 5′–3′) | Reverse primer (name, sequence 5′–3′) | Size |
|---|---|---|---|
| Exon 1 | MYOCe1.L ATCCAGGCACCTCTCAGCAC | MYOCe1.R GCGCCTGTAGCAGGTCACTA | 733 bp |
| Exon 2 | MYOCe2.L AACATAGTCAATCCTTGGGC | MYOCe2.R TTCTGTTCCTCTTCTCCTCC | 341 bp |
| Exon 3 | MYOCe3.L TCACATCTACTGGCTCTGCC | MYOCe3.R CTGGCTGGCTGGCTCTCCCT | 972 bp |
| Exon 3A | MYOCe3.L3 GTCCGCCAGGTTTTTGAGTA | MYOCe3.R3 GGTGCCACAGATGATGAAGG | 423 bp |
| Exon 3B | MYOCe3.L4 GTGCCATTGTCCTCTCCAAA | MYOCe3.R4 CTCTCCCTTCAGCCTGCTC | 395 bp |
| MY5 | MY5.L TCTCTGTCCCTGCTACGTCTT | MY5.R GCACCCATACCCCAATAATAG | ∼239 bp |
| MY3 | MY3.L GTTGGGAGATGTGATTGCAG | MY3.R AGATGGAGGTGGGAAAGTGT | ∼145 bp |