Table 1

PCR primers, reagent concentrations, and thermal cycling conditions used

Primer (source)	Primer sequence	Expected fragment size	PCR reagent formulation*	Thermal cycling profile
*Reagent was from Platinum Taq DNA Polymerase (Invitrogen).
Sars1S/As (Germany)	Sars1S	121 bp	5 μl 10X PCR buffer 1.5 mM MgCl₂ 250 mM of each dNTP 20 μM each primer 2 units of Taq polymerase 2 μl of cDNA 50 μl total volume	Hold: 95°C/3 min
	Sars1S			10 cycles:
	5′-cctctcttgttcttgctcgca-3′			95°C/10 s
	5′-cctctcttgttcttgctcgca-3′			58°C/10 s
	Sar1AS			72°C/20 s
	5′-tatagtgagccgccacacatg-3′			40 cycles:
				95^oC/10 s
				56°C/10 s
				72°C/20 s
				Hold: 72°C/5 min

Cor1/2 (Hong Kong)	Cor1	310 bp		Hold: 94°C/2 min
				10 cycles:
				94°C/30 s
	5′-caccgtttctacaggttagctaacga-3′			56°C/60 s
	Cor2			70°C/45 s
	5′-aaatgtttacgcacggtaagcgtaaaa-3′			35 cycles:
				94°C/30 s
				60°C/45 s
				70°C/45 s
				Hold: 72°C/5 min