Caspase-3 activity of corneal epithelial cells and keratocytes treated with doxycycline
| Sample | Caspase-3 activity (RFU/sec) | ||
|---|---|---|---|
| Doxycycline (μM) | |||
| 0 | 100 | 200 | |
| *Approx 2×105 cells/assay; †approx 5×105 cells/assay. | |||
| RFU, relative fluorescence units. | |||
| Epithelial cells (E)* | 0.46 (0.02) | 0.36 (0.04) | 0.12 (0.02) |
| E + caspase | 2.00 (0.02) | 1.81 (0.00) | 1.23 (0.02) |
| E + caspase inhibitor | 0.01 | 0.01 | 0.01 |
| Caspase | 1.30 | − | − |
| Keratocytes (K)† | 0.02 | 0.01 | 0.01 |
| K + caspase | 0.01 | 0.01 | 0.01 |