Table 1

Values of endothelial cell density in the 100 corneas of the evaluation set

No	Expert 1	Expert 2	Expert 3	Manual average	Manual densities range	% Manual densities range	Automatic	Difference	% Difference
For each cornea, manual densities were obtained by three experts in two 200× images and averaged for each expert (“Expert 1,” “Expert 2,” “Expert 3”). The grand average over all experts was computed (“Manual average”) and the range of the six manual densities was also derived (“Manual densities range”) and expressed as a percentage of the manual average (“% Manual densities range”). Automatic densities were obtained for each cornea in one 100× image by using the proposed technique (“Automatic”) and the difference from the manual average was computed (“Difference”) and expressed also as a percentage of the manual average (“% Difference”).
1	2758	2614	2820	2731	338	12.4	2758	27	1.0
2	2684	2647	2654	2661	216	8.1	2494	−167	−6.3
3	2223	2186	2279	2229	133	6.0	2157	−72	−3.3
4	2189	2078	2174	2147	212	9.9	2216	69	3.2
5	2108	2070	2181	2119	193	9.1	2399	280	13.2
6	2627	2618	2673	2639	104	3.9	2526	−113	−4.3
7	2879	2910	2839	2876	243	8.5	2895	19	0.7
8	2326	2399	2414	2379	180	7.6	2559	179	7.5
9	2082	2076	2102	2087	239	11.5	2186	100	4.8
10	2182	2261	2298	2247	235	10.5	2276	29	1.3
11	1989	1977	2059	2008	123	6.1	2098	90	4.5
12	2115	2048	2171	2111	205	9.7	2276	165	7.8
13	1889	1821	2021	1910	271	14.2	2070	160	8.4
14	1986	1866	1959	1937	241	12.4	2041	104	5.4
15	2619	2560	2633	2604	183	7.0	2690	87	3.3
16	2449	2243	2491	2394	547	22.8	2591	197	8.2
17	1990	1843	1970	1934	204	10.5	1957	23	1.2
18	2130	2044	2197	2123	246	11.6	2041	−82	−3.9
19	2079	1986	2049	2038	136	6.7	2157	119	5.8
20	2268	2222	2395	2295	239	10.4	2216	−79	−3.4
21	2158	2138	2210	2168	149	6.9	2157	−12	−0.5
22	2570	2554	2613	2579	185	7.2	2559	−20	−0.8
23	2248	2219	2263	2243	282	12.6	2431	187	8.4
24	2356	2323	2272	2317	207	8.9	2368	51	2.2
25	2570	2532	2446	2516	269	10.7	2494	−22	−0.9
26	2308	2220	2396	2308	352	15.3	2276	−32	−1.4
27	2328	2158	2220	2235	224	10.0	2307	71	3.2
28	2045	2032	2108	2062	124	6.0	2098	37	1.8
29	2251	2146	2286	2227	195	8.8	2368	141	6.3
30	1939	1945	2061	1981	372	18.8	2157	176	8.9
31	2264	2267	2414	2315	207	8.9	2276	−39	−1.7
32	1710	1650	1758	1706	126	7.4	1692	−14	−0.8
33	1812	1813	2025	1883	228	12.1	2070	186	9.9
34	2307	2152	2209	2223	202	9.1	2399	177	8.0
35	2199	2072	2202	2157	210	9.7	2307	149	6.9
36	2237	2020	2130	2129	280	13.2	2462	333	15.7
37	2025	1980	2113	2039	183	9.0	2307	267	13.1
38	2480	2447	2539	2488	325	13.1	2186	−302	−12.1
39	2348	2268	2375	2330	260	11.2	2337	7	0.3
40	3047	2945	2972	2988	128	4.3	2965	−23	−0.8
41	2900	2875	2838	2871	223	7.8	2792	−79	−2.8
42	2151	2083	2212	2148	192	8.9	2216	68	3.1
43	2078	2025	2053	2052	124	6.0	2098	47	2.3
44	2004	1931	2080	2005	284	14.2	2013	8	0.4
45	2063	2078	2066	2069	125	6.0	2070	1	0.0
46	2317	2275	2339	2310	117	5.1	2494	184	8.0
47	2593	2423	2508	2508	201	8.0	2399	−109	−4.3
48	1976	1995	2000	1990	126	6.3	1929	−61	−3.1
49	2675	2588	2579	2614	312	11.9	2526	−88	−3.4
50	2136	2012	2190	2112	201	9.5	2276	164	7.8
51	2946	2798	2926	2890	201	7.0	2758	−132	−4.6
52	2776	2745	2787	2769	155	5.6	2674	−95	−3.4
53	2922	2806	2829	2852	326	11.4	2826	−26	−0.9
54	2631	2656	2680	2655	151	5.7	2542	−113	−4.3
55	2475	2472	2537	2494	158	6.3	2462	−32	−1.3
56	2266	2295	2334	2298	113	4.9	2353	54	2.4
57	2466	2444	2526	2479	132	5.3	2510	32	1.3
58	2241	2234	2384	2286	242	10.6	2353	67	2.9
59	2326	2327	2436	2363	232	9.8	2337	−25	−1.1
60	2434	2425	2455	2438	223	9.1	2368	−69	−2.8
61	2592	2553	2584	2576	234	9.1	2575	−1	0.0
62	2620	2650	2629	2633	304	11.5	2559	−74	−2.8
63	2057	2090	2123	2090	118	5.6	2186	97	4.6
64	2501	2517	2564	2527	93	3.7	2741	214	8.5
65	2517	2513	2580	2536	130	5.1	2559	22	0.9
66	2395	2389	2431	2405	110	4.6	2526	122	5.1
67	2469	2482	2482	2477	66	2.7	2542	65	2.6
68	2711	2580	2694	2661	230	8.6	2640	−21	−0.8
69	3048	3027	3131	3068	187	6.1	3000	−68	−2.2
70	3042	3087	3166	3098	219	7.1	3072	−26	−0.8
71	2603	2562	2584	2583	83	3.2	2494	−88	−3.4
72	2510	2506	2506	2507	70	2.8	2399	−108	−4.3
73	2237	2233	2256	2242	202	9.0	2201	−41	−1.8
74	2230	2188	2230	2216	78	3.5	2171	−44	−2.0
75	2299	2419	2359	2359	255	10.8	2399	41	1.7
76	2431	2448	2521	2467	125	5.1	2526	60	2.4
77	2167	2207	2214	2196	355	16.2	2261	66	3.0
78	1946	1926	2010	1961	159	8.1	1929	−31	−1.6
79	1810	1899	1896	1868	235	12.6	1971	103	5.5
80	2726	2621	2605	2651	397	15.0	2708	57	2.2
81	2415	2371	2409	2398	111	4.6	2415	17	0.7
82	3027	2812	2880	2906	399	13.7	2809	−97	−3.3
83	3130	2950	3090	3057	210	6.9	2724	−333	−10.9
84	2890	2775	2872	2845	255	9.0	2707	−138	−4.9
85	2715	2542	2575	2610	285	10.9	2641	31	1.2
86	2530	2509	2624	2554	187	7.3	2559	4	0.2
87	2721	2696	2717	2711	107	3.9	2575	−136	−5.0
88	2259	2274	2323	2285	154	6.7	2353	68	3.0
89	2525	2463	2532	2506	115	4.6	2415	−91	−3.6
90	2385	2433	2405	2407	138	5.7	2446	39	1.6
91	2829	2730	2732	2764	152	5.5	2640	−123	−4.5
92	1896	1917	1916	1910	254	13.3	1930	20	1.1
93	2465	2417	2510	2464	259	10.5	2262	−201	−8.2
94	2369	2398	2369	2379	107	4.5	2099	−280	−11.8
95	2448	2383	2336	2389	143	6.0	2384	−5	−0.2
96	2290	2297	2235	2274	143	6.3	2114	−160	−7.0
97	2393	2420	2440	2418	104	4.3	2431	14	0.6
98	2307	2280	2375	2321	169	7.3	2415	95	4.1
99	2462	2519	2426	2469	122	4.9	2462	−7	−0.3
100	2191	2181	2232	2201	365	16.6	2291	90	4.1
Mean	2383	2341	2403	2375	203	8.6	2389	14	0.9
SD	313	304	289	299	84	3.7	261	119	5.1
Min	1710	1650	1758	1706	66	2.7	1692	−333	−12.1
Max	3130	3087	3166	3098	547	22.8	3072	333	15.7