Efficiency of transduction of human corneal endothelium by AdV encoding eGFP
| AdV dose (MOI)* | No | pfu Adv per cornea | Donor age† (years) | Storage time†‡ (days) | Endothelial cell density†§ (cells/mm2) | % eGFP+ cells per cornea†§ | Transductionfailures¶ |
|---|---|---|---|---|---|---|---|
| *MOI: multiplicity of infection; †Mean (SD); ‡Time in cold storage from enucleation until transduction; §As calculated from five central fields (0.15 mm2) on corneal flat mounts by fluorescence microscopy; ¶Transduction failures: fewer than 2% eGFP positive cells/cornea; **p<0.01 compared to very high dose group; ††p<0.05 compared to low and no dose groups. | |||||||
| None | 3 | 0 | 71 (6) | 21 (7) | 1367 (952) | 0 | – |
| Low (10) | 3 | 2×106 | 72 (4) | 25 (3) | 2720 (611)** | 8 (3) | 1 |
| Intermediate (25–100) | 8 | 5×106−2×107 | 68 (10) | 17 (6) | 2195 (558)** | 54 (31) | 0 |
| High (250–1500) | 13 | 5×107−3×108 | 72 (19) | 16 (7) | 2163 (669)** | 78 (31)†† | 1 |
| Very high (10000) | 2 | 2×109 | 65 (8) | 27 (2) | 98 (129) | 64 (26) | 0 |