Antibody fragment yield, purity, and endotoxin contamination following purification (SD)
| Fragment | MW* (kDa) | Yield from 15 litreferment (mg) | Purity | Endotoxin (EU/mg protein) |
|---|---|---|---|---|
| *Determined by size exclusion chromatography. | ||||
| †NA = not applicable: yield of Fab following digestion and purification was 80%. | ||||
| ScFv 20-mer (monomer) | 27 | 145 (32) | >95% | <0.5 |
| ScFv 20-mer (dimer) | 56 | 18 (4) | >95% | <0.5 |
| ScFv 11-mer (dimer) | 52 | 52 (4) | >95% | <0.5 |
| Fab (monomer) | 48 | NA† | >91% | <0.2 |