Profile of antibodies used for ELSIA for VEGF, HGF, IL-1β
| R & D antibodies | Primary antibody concentration (ng/ml) | Secondary capture concentration (ng/ml) | Inter-assayCoV | Intra-assay CoV | Sensitivity |
|---|---|---|---|---|---|
| VEGF-A (isoform 165) | Polyclonal 125 ng/ml | Polyclonal, biotinylated 100 ng/ml | 10% | 3% | 1 pg/ml |
| HGF | Monoclonal, 2000 ng/ml | Biotinylated, 250 ng/ml | 14% | 1% | 5 pg/ml |
| Il-L β | Polyclonal, 2000 ng/ml | Biotinylated, Polyclonal, 200 ng/ml | 17% | 18% | 1 pg/ml |