cyclic 3′5′ guanosine monophosphate fluorescence intensity in human retinal pigment epithelium cells after incubation with different phosphodiesterase inhibitors and guanylyl cyclase stimulation
| Treatment | n | 300–600 | Grey Values | 2700–4095 | |
|---|---|---|---|---|---|
| 600–1500 | 1500–2700 | ||||
| ANP, atrial natriuretic peptide; IBMX, 3-isobutyl-1-methylxanthine; SNP, sodium nitroprusside | |||||
| p<0.001 when compared with control +ANP. | |||||
| Control | 3 | 95 | 5 | 0 | 0 |
| +SNP | 3 | 87 | 13 | 0 | 0 |
| +ANP | 3 | 95 | 5 | 0 | 0 |
| IBMX | 3 | 65 | 24 | 11 | 0 |
| +SNP | 3 | 64 | 34 | 2 | 0 |
| +ANP* | 3 | 22 | 39 | 32 | 7 |
| Sildenafil | 3 | 91 | 9 | 0 | 0 |
| +SNP | 3 | 96 | 4 | 0 | 0 |
| +ANP* | 3 | 18 | 60 | 10 | 12 |
| BAY 60–7550 | 3 | 99 | 1 | 0 | 0 |
| +SNP | 3 | 86 | 14 | 0 | 0 |
| +ANP* | 3 | 01 | 88 | 5 | 6 |
| Sch51866 | 3 | 99 | 1 | 0 | 0 |
| +SNP | 3 | 93 | 7 | 0 | 0 |
| +ANP* | 3 | 40 | 46 | 14 | 0 |