| Herpes virus | Multiplex PCR | Real-time PCR |
| HSV1 | 7/100 (7%) | 7/7 (100%) |
| HSV2 | 3/100 (3%) | 3/3 (100%) |
| VZV | 29/100 (29%) | 21/29 (72%) |
| EBV | 19/100 (19%) | 6/19 (32%) |
| CMV | 6/100 (6%) | 4/6 (67%) |
| HHV6 | 1/100 (1%) | 1/1 (100%) |
| HHV7 | 0/100 (0%) | – |
| HHV8 | 0/100 (0%) | – |
| Total | 65/100 (65%) | 42/65 (65%) |
Qualitative multiplex PCR was performed in order to screen for and detect human herpes virus (HHV) genomic DNA, HHV1–HHV8. When the genomic DNA was detected by the multiplex PCR (n = 65), real-time PCR was then performed only for the HHV.
CMV, cytomegalovirus; EBV, Epstein–Barr virus; HSV, herpes simplex virus; VZV, Varicella-zoster virus.