Group | Supplement |
1 | — |
2 | 10% FCS (Invitrogen, Paisley, UK) |
3 | α-amylase (93 IU/l, Sigma-Aldrich, Poole, UK) |
4 | rhEGF (1 μg/ml) |
5 | rhEGF (10 μg/ml) |
6 | Dextrin–rhEGF (1 μg/ml rhEGF equivalent in PBS) |
7 | Dextrin–rhEGF (1 μg/ml rhEGF equivalent in PBS) and α-amylase (93 IU/l) (pre-incubated for 24 h at 37°C) |
8 | Dextrin–rhEGF (10 μg/ml rhEGF equivalent in PBS) and α-amylase (93 IU/l) (pre-incubated for 24 h at 37°C) |
FCS, fetal calf serum; PBS, phosphate buffered saline; rhEGF, recombinant human epidermal growth factor.