Human primers for real-time PCR
| Primer | Sequence (5′ to 3′) | Product size |
|---|---|---|
| TGFβ2 | Forward GTTCGATTTGACGTCTCAGCAAT | 107 |
| Reverse CAATCCGTTGTTCAGGCACTCT | ||
| CTGF | Forward CTCGCGGCTTACCGACTG | 169 |
| Reverse GGCTCTGCTTCTCTAGCCTG | ||
| CNTF | Forward CGTTGGAGACATACACAAATGG | 126 |
| Reverse AGAACCCTGAAGTGGAAGGAC | ||
| VEGF | Forward ACTGAGGAGTCCAACATCAC | 130 |
| Reverse CTTGTCTTGCTCTATCTTTC | ||
| GAPDH | Forward GAGTCCACTGGCGTCTTCAC | 120 |
| Reverse GTTCACACCCATGACGAACA |
CNTF, ciliary neurotrophic factor; CTGF, connective tissue growth factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TGF, transforming growth factor; VEGF, vascular endothelial growth factor.