Details of antibody and stain concentrations
| Antibody/stain | Supplier | Concentration |
| Zonula Occludens-1 (ZO-1) (mouse) | Thermo Fisher Scientific (40–2200) | 2.5 µg/mL |
| Na+K+ATPase (rabbit) | Santa Cruz (sc-71638) | 5 µg/mL |
| Goat antirabbit Alexa Fluor 488 secondary | Thermo Fisher Scientific (A11008) | 4 µg/mL |
| Goat antimouse Alexa Fluor 488 secondary | Thermo Fisher Scientific (A11001) | 4 µg/mL |
| Alexa Fluor 488 phalloidin | Thermo Fisher Scientific (A12379) | 2 units/mL |
| DAPI dilactate | Thermo Fisher Scientific (D3571) | 300 nM |
DAPI, 4’,6-diamidino-2-phenylindole.