The threshold-free cluster-weighted (TFCW) method revealed 12 white matter tracts with different generalised fractional anisotropy (GFA) values between amblyopic adults and normal-sighted controls
| Tract No. | Name of tracts | GFA values Mean± SD | P value | |
| Controls | Amblyopia | |||
| 1 | Left optic radiation | 0.659±0.038 | 0.623±0.038 | 0.0176 |
| 2 | Right optic radiation | 0.654±0.055 | 0.594±0.044 | 0.0038 |
| 3 | Left IFOF | 0.717±0.026 | 0.682±0.047 | 0.0072 |
| 4 | Left arcuate fasciculus | 0.643+0.026 | 0.598±0.044 | 0.0022 |
| 5 | Left TR of the auditory nerve | 0.371±0.030 | 0.342±0.030 | 0.0110 |
| 6 | CF of the middle temporal gyrus | 0.161±0.041 | 0.128±0.018 | 0.0111 |
| 7 | Left frontal aslant tract | 0.609±0.054 | 0.554±0.043 | 0.0063 |
| 8 | Left fornix | 0.560±0.079 | 0.475±0.088 | 0.0102 |
| 9 | CF of the genu | 0.592±0.037 | 0.539±0.042 | 0.0007 |
| 10 | Left uncinate fasciculus | 0.482±0.050 | 0.520±0.045 | 0.0022 |
| 11 | Right uncinate fasciculus | 0.341±0.033 | 0.390±0.068 | 0.0056 |
| 12 | Left FS of the OFC | 0.346±0.048 | 0.311±0.046 | 0.0720 |
CF, callosal fibres; FS, frontal striatum; IFOF, inferior fronto-occipital fasciculus; OFC, orbitofrontal cortex; TR, thalamic radiation.