Studies evaluating real-time PCR assays for detection of Toxoplasma gondii DNA in intraocular samples using the B1 gene and Rep529 targets
| Author | Year | Country | Single-reaction | Internally controlled | Sample size | Type of fluid | Sensitivity n/N (%) | Specificity (%) | Reference test | ||
| B1 | Rep529 | B1 | Rep529 | ||||||||
| Cassaing15 | 2006 | France | No | No | 3 | AH:3 | 3/3 (100%) | 3/3 (100%) | NA | NA | Rep529 real-time PCR |
| Fekkar et al10 | 2008 | France | No | Yes | 110 | AH:92; VF: 18 | 9/34 (26.5%) | 13/34 (38.2%) | 76/76 (100%) | 76/76 (100%) | Ophthalmological examination |
| Belaz et al13 | 2015 | France | No | Yes | 6 | AH:5; VF:1 | 5/6 (83.3%) | 6/6 (100%) | NA | NA | Western blot |
| Santos et al16 | 2015 | Brazil | No | Yes | 43 | AH:43 | 16/43 (100%) | 16/43 (100%) | NA | NA | Fundoscopic examination compatible with active focal necrotizing retinochoroiditis |
| Gomez | CR | USA | Yes | Yes | 148 | AH:16; VF:132 | 35*/36 (97.2%) | 35*/36 (97.2%) | 112/112 (100%) | 111*/112 (99%) | Conventional nested B1 PCR |
*There were two discrepant samples; one dual-target negative, nested B1 positive, and the other dual-target positive (Rep529 only; Ct value=39.14), nested B1 negative. Reference testing at the Palo Alto Medical Foundation Toxoplasmosis Serology Laboratory revealed that both specimens were negative for T. gondii DNA using B1 real-time PCR.
AH, aqueous humour; CR, current report; NA, not available; VF, vitreous fluid.