Skip to main content
SpringerLink
Log in

Kinematics of epithelial wound closure in the rabbit cornea

  • Published:
Documenta Ophthalmologica Aims and scope Submit manuscript

Abstract

The kinematics of wound closure in the healing rabbit corneal epithelium are modelled. The analysis is able to model the movement of the wound edge on the corneal surface. A unified formalism is described which embeds the surface velocity of the wound margin within the computational algorithm to enable calculation of the surface velocity of the wound margin from the time course of (1) planar wound area, or (2) wound diameter. The procedure can be implemented with the standard least-squares estimation procedure. A polynomial velocity function is discussed, but the model allows for any appropriate velocity function to be used to suit particular conditions of wound closure. Corneal curvature effects are incorporated to avoid the errors associated with a planar representation of wound closure on a curved corneal surface. The approach offers greater flexibility by avoiding the theoretical flaws of previous models which are often limited in scope and nonbiological in nature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

r.m.s.:

root mean square

CVM:

constant velocity model

References

  1. Arey LB, Covode WM. The method of repair in epithelial wounds of the cornea. Anat Rec 1943; 86: 75–86.

    Google Scholar 

  2. Friedenwald JS, Buschke W. The influence of some experimental variables on the epithelial movements in the healing of corneal wounds. J Cell Comp Physiol 1944; 23: 95–107.

    Google Scholar 

  3. Friedenwald JS, Buschke W. Mitotic and wound-healing activities of the corneal epithelium. Arch Ophthalmol 1944; 32: 410–13.

    Google Scholar 

  4. Friedenwald JS, Buschke W. Some factors concerned in the mitotic and wound-healing activities of the corneal epithelium. Trans Am Ophthalmol Soc 1944; 42: 371–83.

    Google Scholar 

  5. Hara J. An autoradiographic study of regeneration of corneal epithelium. Folia Ophthalmol Jpn 1962; 13: 443–66.

    Google Scholar 

  6. Du Nouy PL. Cicatrization of wounds, II: Mathematical expression of the curve representing cicatrization. J Exp Med 1916; 24: 451–60.

    Google Scholar 

  7. Du Nouy PL. Cicatrization of wounds, III: The relation between the age of the patient, the area of the wound, and the index of cicatrization. J Exp Med 1916; 24: 461–70.

    Google Scholar 

  8. Du Nouy PL. Biological time. New York: Macmillan, 1937.

    Google Scholar 

  9. Arey LB. Wound healing. Physiol Rev 1936; 16: 327–406.

    Google Scholar 

  10. Block P, Seiter I, Oehlert W. Autoradiographic studies of the initial cellular response to injury. Exp Cell Res 1963; 30: 311–21.

    Google Scholar 

  11. Harris TM, Sheppard LB, Shanklin WM, Fox RR. A comparison of corneal epithelium regeneration in normal and buphthalmic rabbits. Invest Ophthalmol 1970; 9: 122–30.

    Google Scholar 

  12. Ho PC, Davis WH, Elliot JH Cohen S. Kinetics of corneal epithelial regeneration of and epidermal growth factor. Invest Ophthalmol 1974; 13: 804–9.

    Google Scholar 

  13. Moses RA, Parkison G, Schuchardt R. A. standard large wound of the corneal epithelium in the rabbit. Invest Ophthalmol Vis Sci 1979; 18: 103–6.

    Google Scholar 

  14. Thompson P, Desbordes JM, Giraud J, Pouliquen Y, Barritault D, Courtois Y. The effect of eye derived growth factor (EDGF) on corneal epithelial regeneration. Exp Eye Res 1982; 34: 191–99.

    Google Scholar 

  15. Ubels JL, Edelhauser HF, Austin KH. A comparison of healing of corneal epithelial wounds stained with fluorescein or Richardson's stain. Invest Ophthalmol Vis Sci 1982; 23: 127–31.

    Google Scholar 

  16. Datiles MB, Kador PF, Fukui HN, Hu T-S, Kinoshita JH. Corneal re-epithelialization in galactosemic rats. Invest Ophthalmol Vis Sci 1983; 24: 563–69.

    Google Scholar 

  17. Kwok LS. Calculation and application of the anterior surface area of a model human cornea. J Theor Biol 1984; 108: 295–313.

    Google Scholar 

  18. Langston RHS, Pavan-Langston D, Dohlman CH. Antiviral medication and corneal wound healing. Arch Ophthalmol 1974; 92: 509–13.

    Google Scholar 

  19. Foster CS, Pavan-Langston D. Corneal wound healing and antiviral medication. Arch Ophthalmol 1977; 95: 2062–67.

    Google Scholar 

  20. Lass JH, Pavan-Langston D, Park N-H. Aciclovir and corneal wound healing. Am J Ophthalmol 1979; 88: 102–8.

    Google Scholar 

  21. Foster CS, Lass JH, Moran-Wallace K, Giovanoni R. Ocular toxicity of topical fungal agents. Arch Ophthalmol 1981; 99: 1081–84.

    Google Scholar 

  22. Buschke W. Morphologic changes in cells of corneal epithelium in wound healing. Arch Ophthalmol 1949; 41: 306–16.

    Google Scholar 

  23. Davis FA. The anatomy and histology of the eye and orbit of the rabbit. Trans Am Ophthalmol Soc 1929; 27: 410–41.

    Google Scholar 

  24. Prince JH. The rabbit in eye research. Springfield: Thomas, 1964.

    Google Scholar 

  25. Lindmark R, Edelhauser HF, McCarey BE. Design and fitting of rabbit hydrophilic contact lenses. Contact Lens Soc Am J 1972; 6: 21–27.

    Google Scholar 

  26. Murphy GM. Ordinary differential equations and their solutions. New York: Van Nostrand, 1960.

    Google Scholar 

  27. Pfister RR. The healing of corneal epithelial abrasions in the rabbit: a scanning electron microscope study. Invest Ophthalmol 1975; 14: 648–61.

    Google Scholar 

  28. Pfister RR, Burstein N. The alkali burned cornea, I: Epithelial and stromal repair. Exp Eye Res 1976; 23: 519–35.

    Google Scholar 

  29. Scheid F. Schaum's outline of theory and problems of numerical analysis. New York: McGraw-Hill, 1968.

    Google Scholar 

  30. Sorsby A, Sheridan M. Changes in the refractive power of the cornea during growth: observations on the rabbit. Br J Ophthalmol 1953; 37: 555–57.

    Google Scholar 

  31. Stone J, Leary G. Changes in refraction during growth: the significance of changes in corneal power as observed in the rabbit. Br J Physiol Optics 1957; 14: 90–94.

    Google Scholar 

  32. MacKeen D, Labaw B. A method of fitting soft contact hydrophilic lenses to rabbits for long-term experiments. Int Contact Lens Clin 1978; 5: 190–3.

    Google Scholar 

  33. Heydenreich A. Die Zellmigration im Bereich der Hornhaut. Ber Versamm Dtsch Ophthalmol Gessellsch 1959; 62: 287–94.

    Google Scholar 

  34. Kuwabara T, Perkins DG, Cogan DG. Sliding of the epithelium in experimental corneal wounds. Invest Ophthalmol 1976; 15: 4–14.

    Google Scholar 

  35. Brewitt H. Sliding epithelium in experimental corneal wounds: a scanning electron microscope study. Acta Ophthalmol 1979; 57: 945–58.

    Google Scholar 

  36. Yamanaka H, Eguchi G. Regeneration of the cornea in adult newts: overall process and behavior of epithelial cells. Differentiation 1981; 19: 84–92.

    Google Scholar 

  37. Kwok LS, Madigan M. Application of a new kinematic model of epithelial wound closure in the cat cornea [Abstract]. Invest Ophthalmol Vis Sci 1985; 26 (suppl): 91.

    Google Scholar 

  38. Kwok LS, Madigan M. A new analysis of wound closure kinematics in the cat and rabbit corneal epithelium. Clin Exp Optom 1986; 69: 4–12.

    Google Scholar 

  39. Hatze H. A method for describing the motion of biological systems. J Biomech 1976; 9: 101–104.

    Google Scholar 

  40. Guzman-Llansa L, Diez-Noguera A, Igual A, Rosich M. Circadian rhythm of corneal re-epithelialization in the rabbit. IRCS Med Sci 1986; 14: 798–99.

    Google Scholar 

  41. Collin HB, Grabsch BE. The effect of ophthalmic preservatives on the healing rate of the rabbit corneal epithelium after keratectomy. Am J Optom Physiol Opt 1982; 59: 215–22.

    Google Scholar 

  42. Grant WM. Toxicology of the eye. Springfield: Thomas, 1974.

    Google Scholar 

  43. Bron AJ, McLendon B, Buckley CA, Tripathi RC. Retardation of corneal resurfacing. In: Trevor-Roper PD, ed. The cornea in health and disease (VIth congress of the European Society of Ophthalmology). Royal Society of medicine international congress and symposium series No. 40. London: Academic Press and the Royal Society of Medicine, 1981: 203–208.

    Google Scholar 

  44. Putt FA. Manual of histopathological staining methods. New York: Wiley, 1972. p. 100.

    Google Scholar 

  45. De Ocampo G, Sunga RN. Studies on the epithelization of corneal grafts with methylene blue. Am J Ophthalmol 1963; 56: 801–806.

    Google Scholar 

  46. Cintron C, Hassinger L, Kublin CL, Friend J. A simple method for the removal of rabbit corneal epithelium utilizing n-heptanol. Ophthalmic Res 1979; 11: 90–96.

    Google Scholar 

  47. Jumblatt MM, Fogle JA, Neufeld AH. Cholera toxin stimulates adenosine 3′,5′-monophosphate synthesis and epithelial wound closure in the rabbit cornea. Invest Ophthalmol Vis Sci 1980; 19: 1321–27.

    Google Scholar 

  48. Friend J, Kiorpes TC, Thoft RA. Diabetes mellitus and the rabbit corneal epithelium. Invest Ophthalmol Vis Sci 1981; 21: 317–21.

    Google Scholar 

  49. Ubels JL, Edelhauser HF, Shaw D. Measurement of corneal epithelial healing rates and corneal thickness for evaluation of ocular toxicity of chemical substances. J Toxicol Cut Ocular Toxicol 1982; 1: 133–45.

    Google Scholar 

  50. Matsumoto S. Contribution to the study of epithelial movement: the corneal epithelium of the frog in tissue culture. J Exp Zool 1918; 26: 545–63.

    Google Scholar 

  51. Buck RC. Cell migration in repair of mouse corneal epithelium. Invest Ophthalmol Vis Sci 1979; 18: 767–84.

    Google Scholar 

  52. Bernat R. The role of adrenergic and cholinergic mediator and 5-HT in the regeneration of corneal epithelium (in Polish). Acta Physiol Pol 1968; 19: 191–203.

    Google Scholar 

  53. Lash JW. Studies on wound closure in urodeles. J Exp Zool 1955; 128: 13–28.

    Google Scholar 

  54. Petroutsos G, Guimaraes R, Giraud JP, Renard G, Pouliquen Y. Effect of acetylcysteine (Mucomyst) on epithelial wound healing. Ophthalm Res 1982; 14: 241–48.

    Google Scholar 

  55. Petroutsos G, Guimaraes R, Giraud JP, Pouliquen Y. Antibiotics and corneal epithelial wound healing. Arch Ophthalmol 1983; 101: 1775–78.

    Google Scholar 

  56. Leopold IH. Local toxic effect of detergents on ocular structures. Arch Ophthalmol 1945; 34: 99–102.

    Google Scholar 

  57. Holzbauer M, Rigler R. Electric shock and the regeneration of the corneal epithelium of rabbits. Nature 1951; 168: 919.

    Google Scholar 

  58. Shimoda S. Influence of low temperature upon corneal regeneration (in Japanese). Acta Soc Ophthalmol Jpn 1952; 56: 1222–29.

    Google Scholar 

  59. Frati L, Daniele S, Delogu A, Covelli I. Selective binding of the epidermal growth factor and its specific effects on the epithelial cells of the cornea. Exp Eye Res 1972; 14: 135–41.

    Google Scholar 

  60. Rucker I, Kettrey R, Bach F, Zeleznick L. A safety test for contact lens wetting solutions: evaluation of commercially available solutions. Ann Ophthalmol 1972; 4: 1000–6.

    Google Scholar 

  61. Kahan A, Hammer H. Pindolol in the treatment of corneal disorders. In: Trevor-Roper PD, ed. The cornea in health and disease (VIth congress of the European Society of Ophthalmology). Royal Society of medicine international congress and symposium series No. 40. London: Academic Press and the Royal Society of Medicine, 1981: 1073–75.

    Google Scholar 

  62. Maudgal PC, De Kimpe N, De Clercq E, Descamps J, Missotten L, Geysen A. Influence of (E)-5-(2-bromovinyl)-2′-deoxyuridine on corneal epithelium healing. Graefes Arch Clin Exp Ophthalmol 1982; 218: 275–81.

    Google Scholar 

  63. Gipson IK, Kiorpes TC, Brennan SJ. Epithelial sheet movement: effects of tunicamycin on migration and glycoprotein. Dev Biol 1984; 101: 212–20.

    Google Scholar 

  64. MacRae SM, Brown B, Edelhauser HF. The corneal toxicity of presurgical skin antiseptics. Am J Ophthalmol 1984; 97: 221–32.

    Google Scholar 

  65. Nork TM, Holly FJ, Hayes J, Wentlandt T, Lamberts DW. Timolol inhibits corneal epithelial wound healing in rabbits and monkeys. Arch Ophthalmol 1984; 102: 1224–28.

    Google Scholar 

  66. Hatchell DL, Magolan JJ, Besson MJ, Goldman AI, Pederson HJ, Schultz KJ. Damage to the epithelial basement membrane in the corneas of diabetic rabbits. Arch Ophthalmol 1983; 101: 469–71.

    Google Scholar 

  67. Schaeffer AJ. An attempt to influence corneal wound healing by local application of certain amino acids. Am J Ophthalmol 1950; 33: 741–50.

    Google Scholar 

  68. Payrau P, Dohlman CH. IDU in corneal wounding healing. Am J Ophthalmol 1964; 57: 999–1002.

    Google Scholar 

  69. Krishna N, Brow F. Polyvinyl alcohol as an ophthalmic vehicle. Am J Ophthalmol 1964; 57: 99–106.

    Google Scholar 

  70. Srinivasan BD, Kulkarni PS. The effect of steroidal and nonsteroidal anti-inflammatory agents on corneal reepithelialization. Invest Ophthalmol Vis Sci 1981; 20: 688–90.

    Google Scholar 

  71. Srinivasan BD. Corneal reepithelialization and anti-inflammatory agents. Trans Am Ophthalmol Soc 1982; 80: 758–822.

    Google Scholar 

  72. Bowman KA, Green K. Corneal epithelial healing rates after topical nucleotides. Curr Eye Res 1982; 1: 619–22.

    Google Scholar 

  73. Jumblatt MM, Neufeld AH. Characterization of cyclic AMP-mediated wound closure of the rabbit corneal epithelium. Curr Eye Res 1981; 1: 189–95.

    Google Scholar 

  74. Schwartz SM, Stemerman MB, Benditt EP. The aortic intima, II: Repair of the aortic lining after mechanical denudation. Am J Pathol 1975; 81: 15–42.

    Google Scholar 

  75. Wong MKK, Gotlieb AI. In vitro reendothelialization of a single-cell wound: role of microfilament bundles in rapid lamellipodia-mediated wound closure. Lab Invest 1984; 51: 75–81.

    Google Scholar 

  76. Hanna C, Fraunfelder FT, Cable M, Hardberger RE. The effect of ophthalmic ointments on corneal wound healing. Am J Ophthalmol 1973; 76: 193–200.

    Google Scholar 

  77. Snowden JM. Wound contraction: a quantitative interpretation. Aust J Exp Biol Med Sci 1981; 59: 203–17.

    Google Scholar 

  78. Snowden JM. Wound closure: an analysis of the relative contributions of contraction and epithelialization. J Surg Res 1984; 37: 453–63.

    Google Scholar 

  79. Crosson CE, Klyce SD, Beuerman RW. Epithelial wound closure in the rabbit cornea. Invest Ophthalmol Vis Sci 1986; 27: 464–473.

    Google Scholar 

  80. Shimoda S. An experimental study on the influence of cold upon the healing of injuries to the eye (in Japanese). Acta Soc Ophthalmol Jpn 1951; 55: 1216–39.

    Google Scholar 

  81. Crabb CV. Endocrine influences on ulceration and regeneration in the alkali-burned cornea. Arch Ophthalmol 1977; 95: 1866–70.

    Google Scholar 

  82. Monod J, Wyman J, Changeux, J-P. On the nature of allosteric transitions: a plausible model. J Molec Biol 1965; 12: 88–118.

    Google Scholar 

  83. Robin JB, Kash RL, Azen SP, Schanzlin DJ. Lack of effect of pilocarpine on corneal epithelial wound healing. Curr Eye Res 1984; 3: 403–6.

    Google Scholar 

  84. Petroutsos G, Courty J, Guimaraes R, Pouliquen Y, Barritault D, Plouët J, Courtois Y. Comparison of the effects of EGF, pFGF and EDGF on corneal epithelium wound healing. Curr Eye Res 1984; 3: 593–98.

    Google Scholar 

  85. Lumière A. Les lois de la cicatrisation des plaies cutanées. Rev Chir 1917; 53: 656–678.

    Google Scholar 

  86. Lumière A. A propos des lois de la cicatrisation: discussion des résultats, Rev Chir 1918; 55: 549–565.

    Google Scholar 

  87. Fauré-Frémiet E, Vlès F. Les lois de la cicatrisation des plaies sont-elles réductibles aux lois générales de croissance des organismes? Compt Rend Hebdom Acad Paris 1919; 168: 363–6.

    Google Scholar 

  88. Howes EL. The rate and nature of epithelization in wounds with loss of substance. Surg Gynecol Obstet 1943; 76: 738–45.

    Google Scholar 

  89. Van Den Brenk HAS. Studies in restorative growth processes in mammalian wound healing. Br J Surg 1956; 43: 525–50.

    Google Scholar 

  90. Chan KY, Patton DL, Cosgrove YT. Time-lapse videomicroscopic study of in vitro wound closure in rabbit corneal cells. Invest Ophthalmol Vis Sci 1989; 30: 2488–2498.

    Google Scholar 

  91. Simmons SJ, Jumblatt MM, Neufeld AH. Corneal epithelial wound closure in tissue culture: an in vitro model of ocular irritancy. Toxicol Appl Pharmacol 1987; 88: 13–23.

    Google Scholar 

  92. Daniele S, Frati L, Fiore C, Santoni G. The effect of the epidermal growth factor (EGF) on the corneal epithelium in humans. Graefes Arch Klin Exp Ophthalmol 1979; 210: 159–65.

    Google Scholar 

  93. Moses RA, Brown DN. Short-term re-epithelialization of the alkali-burned rabbit cornea. Ann Ophthalmol 1985; 17: 714–16.

    Google Scholar 

  94. Chung J.-H. Healing of rabbit corneal alkali wounds in vitro. Cornea 1990; 9: 36–40.

    Google Scholar 

  95. Nelson JD, Silverman V, Lima PH, Beckman G. Corneal epithelial wound healing: a tissue culture assay on the effects of antibiotics. Curr Eye Res 1990; 9: 277–285.

    Google Scholar 

  96. Ormerod LD, Garsd A, Reddy CV, Gomes SA, Abelson MB, Kenyon KR. Dynamics of corneal epithelial healing after an alkali burn: a statistical analysis. Invest Ophthalmol Vis Sci 1989; 30: 1784–93.

    Google Scholar 

  97. Tuft S, Eggli P, Boulton M, Marshall J. Assessment of corneal wound repair in vitro. Curr Eye Res 1989; 8: 713–19.

    Google Scholar 

  98. Fredj-Reygrobellet D, Plouët J, Delayre Th, Baudouin Ch, Bourret F, Lapalus Ph. Effects of aFGF and bFGF on wound healing in rabbit corneas. Curr Eye Res 1987; 6: 1205–9.

    Google Scholar 

  99. Brazzell RK, Stern ME, Aquavella JV, Beuerman RW, Baird L. Human recombinant epidermal growth factor in experimental corneal wound healing. Invest Ophthalmol Vis Sci 1991; 32: 336–40.

    Google Scholar 

  100. Obenberger J. Paper strips and rings as simple tools for standardization of experimental eye injuries. Ophthalmic Res 1975; 7: 363–67.

    Google Scholar 

  101. Ho PC, Elliot JH. Kinetics of corneal epithelial regeneration, II: Epidermal growth factor and topical corticosteroids. Invest Ophthalmol 1975; 14: 630–33.

    Google Scholar 

  102. Shaw D, Bistner S, Edelhauser HF, Gorlich J. Rate of corneal re-epithelialization measured by planimetry and change in corneal thickness measured by pachometry in the dog, cat, rabbit, guinea pig, rat and primate. Transactions of the 12th annual scientific program of the College of Veterinary Ophthalmologists 1981; 1–20.

  103. Matsuda M, Ubels JL, Edelhauser HF. A larger corneal epithelial wound closes at a faster rate. Invest Ophthalmol Vis Sci 1985; 26: 897–900.

    Google Scholar 

  104. Boisjoly HM, Beaulieu A, Giasson M, Ménard C. The effect of fibronectin compared to albumin on rabbit corneal epithelial wound healing [Abstract]. Invest Ophthalmol Vis Sci 1987; 28 (suppl): 52.

    Google Scholar 

  105. Medgyaszay A, Hegedüs J. On the epithelialization of the cornea. Szemeszet 1967; 104: 66–69.

    Google Scholar 

  106. Dua HS, Forrester JV. Clinical patterns of corneal epithelial wound healing. Am J Ophthalmol 1987; 104: 481–89.

    Google Scholar 

  107. Dua HS, Forrester JV. The corneoscleral limbus in human corneal epithelial wound healing. Am J Ophthalmol 1990; 110: 646–56.

    Google Scholar 

  108. Hill RM, Lowther GE. Oxygen flux across the tear-epithelial interface as an index of corneal wound repair. Am J Optom Arch Am Acad Optom 1967; 44: 267–75.

    Google Scholar 

  109. Bracher R. Radioautographic analysis of the synthesis of protein, RNA, DNA, and sulphated mucopolysaccharides in the early stages of corneal wound healing. Invest Ophthalmol 1967; 6: 565–73.

    Google Scholar 

  110. Gipson IK, Kiorpes TC. Epithelial sheet movement: protein and glycoprotein synthesis. Dev Biol 1982; 92: 259–62.

    Google Scholar 

  111. Hayashi K, Cheng H-M, Iwasaki M, Xiong H, Kenyon KR. Metabolic analysis of reepithelializing rabbit cornea using phosphorus-31 nuclear magnetic resonance spectroscopy. Graefes Arch Clin Exp Ophthalmol 1990; 228: 73–77.

    Google Scholar 

  112. Sherratt JA, Murray JD. Models of epidermal wound healing. Proc Royal Soc Lond B 1990; 241: 29–36.

    Google Scholar 

  113. Srinivasan BD, Worgul BV, Iwamoto T, Eakins KE. The reepithelialization of rabbit cornea following partial and complete epithelial denudation. Exp Eye Res 1977; 25: 343–51.

    Google Scholar 

  114. Friend J, Thoft RA. Functional competence of regenerating ocular surface epithelium. Invest Ophthalmol Vis Sci 1978; 17: 134–39.

    Google Scholar 

  115. Essepian JP, Wei F, Hildesheim J, Jester JV. Comparison of corneal epithelial wound healing rates in scrape vs. lamellar keratectomy injury. Cornea 1990; 9: 294–98.

    Google Scholar 

  116. Thompson HW, Malter JS, Steinemann TL, Beuerman RW. Flow cytometry measurements of the DNA content of corneal epithelial cells during wound healing. Invest Ophthalmol Vis Sci 1991; 32: 433–36.

    Google Scholar 

  117. Kitazawa T, Kinoshita S, Fujita K, Araki K, Watanabe H, Ohashi Y, Manabe R. The mechanism of accelerated corneal epithelial healing by human epidermal growth factor. Invest Ophthalmol Vis Sci 1990; 31: 1773–78.

    Google Scholar 

  118. Pullinger BD, Mann I. Avascular healing in the cornea. J Pathol Bact 1943; 55: 151–58.

    Google Scholar 

  119. Mann I. A study of epithelial regeneration in the living eye. Br J Ophthalmol 1944; 28: 26–40.

    Google Scholar 

  120. Friedenwald JS. Growth pressure and metaplasia of conjunctival and corneal epithelium. Documenta Ophthalmol 1951; 5–6: 184–92.

    Google Scholar 

  121. Sheppard LB, Shanklin WM, Harris TM, Fox RR. A histological study of regenerating epithelium of normal and buphthalmic rabbit cornea. Ophthalmic Res 1971; 2: 116–25.

    Google Scholar 

  122. Thoft RA, Friend J. Biochemical transformation of regenerating ocular surface epithelium. Invest Ophthalmol Vis Sci 1977; 16: 14–20.

    Google Scholar 

  123. Shapiro MS, Friend J, Thoft RA. Corneal reepithelialization from the conjunctiva. Invest Ophthalmol Vis Sci 1981; 21: 135–42.

    Google Scholar 

  124. Kinoshita S, Kiorpes TC, Friend J, Thoft RA. Limbal epithelium in ocular surface wound healing. Invest Ophthalmol Vis Sci 1982; 23: 73–80.

    Google Scholar 

  125. Chen JJY, Tseng SCG. Corneal epithelial wound healing in partial limbal deficiency. Invest Ophthalmol Vis Sci 1990; 31: 1301–14.

    Google Scholar 

  126. Srinivasan BD, Eakins KE. The reepithelialization of rabbit cornea following single and multiple denudation. Exp Eye Res 1979; 29: 595–600.

    Google Scholar 

  127. Fukushi S, Merola LO, Tanaka M, Datiles M, Kinoshita JH. Reepithelialization of denuded corneas in diabetic rats. Exp Eye Res 1980; 31: 611–21.

    Google Scholar 

  128. Huang AJW, Tseng SCG. Corneal epithelial wound healing in the absence of limbal epithelium. Invest Ophthalmol Vis Sci 1991; 32: 96–105.

    Google Scholar 

  129. Miyata K, Murao M, Sawa M, Tanishima T. New wound-healing model using cultured corneal endothelial cells, 1: Quantitative study of healing process. Jpn J Ophthalmol 1990; 34: 257–66.

    Google Scholar 

  130. Miyata K, Murao M, Sawa M, Tanishima T. New wound-healing model using cultured bovine corneal endothelial cells, 2: Role of migration and mitosis studied by immunohistochemistry. Jpn J Ophthalmol 1990; 34: 267–74.

    Google Scholar 

  131. Weimar VL, Squires EL, Knox RJ. Acceleration of healing of rabbit corneal endothelium by mesodermal growth factor. Invest Ophthalmol Vis Sci 1980; 19: 350–61.

    Google Scholar 

  132. Matsuda M, Sawa M, Edelhauser HF, Bartels SP, Neufeld AH, Kenyon KR. Cellular migration and morphology in corneal endothelial wound repair. Invest Ophthalmol Vis Sci 1985; 26: 443–49.

    Google Scholar 

  133. Binder RF, Binder HF. Regenerative process in the endothelium of the cornea. Arch Ophthalmol 1957; 57: 11–13.

    Google Scholar 

  134. Morton PL, Ormsby HL, Basu PK. Healing of endothelium and Descemet's membrane of rabbit cornea. Am J Ophthalmol 1958; 46: 62–67.

    Google Scholar 

  135. Chi HH, Teng CC, Katzin HM. Healing process in the mechanical denudation of the corneal endothelium. Am J Ophthalmol 1960; 49: 693–703.

    Google Scholar 

  136. Yano M, Tanishima T. Wound healing in rabbit corneal endothelium. Jpn J Ophthalmol 1980; 24: 297–309.

    Google Scholar 

  137. Ubels JL, Edelhauser HF. Healing of corneal epithelial wounds in marine and freshwater fish. Curr Eye Res 1983; 2: 613–19.

    Google Scholar 

  138. Honda H, Ogita Y, Higuchi S, Kani K. Cell movements in a living mammalian tissue: long-term observation of individual cells in wounded corneal endothelia of cats. J Morphol 1982; 174: 25–39.

    Google Scholar 

  139. Schwartz SM, Haudenschild CC, Eddy EM. Endothelial regeneration, I: Quantitative analysis of initial stages of endothelial regeneration in rat aortic intima. Lab Invest 1978; 38: 568–80.

    Google Scholar 

  140. Vesely P, Weiss RA. Cell locomotion and contact inhibition of normal and neoplastic rat cells. Int J Cancer 1973; 11: 64–76.

    Google Scholar 

  141. Tsubouchi S. Kinetic analysis of epithelial cell migration in the mouse descending colon. Am J Anat 1981; 161: 239–46.

    Google Scholar 

  142. Tsubouchi S. Theoretical implications for cell migration through the crypt and the villus of labelling studies conducted at each position within the crypt. Cell Tissue Kinet 1983; 16: 441–56.

    Google Scholar 

  143. Newgreen DF, Ritterman M, Peters EA. Morphology and behaviour of neural crest cells of chick embryo in vitro. Cell Tissue Res 1979; 203: 115–40.

    Google Scholar 

  144. Kwok LS. Analysis of re-epithelialization: the pitfalls and fallacies. A general kinematic model for wound healing studies [Poster at 8th International Congress for Eye Research, San Francisco, 6–7 Sept. 1988.]

Download references

Author information

Authors and Affiliations

  1. Corneal Physiology and Biophysics Laboratory, College of Optometry,University of Houston, 77204-6052, Houston, TX, U.S.A.

    L. Stephen Kwok

Authors
  1. L. Stephen Kwok
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. Stephen Kwok.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kwok, L.S. Kinematics of epithelial wound closure in the rabbit cornea. Doc Ophthalmol 77, 1–38 (1991). https://doi.org/10.1007/BF00154875

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00154875

Key words

Search

Navigation