Summary
To see whether or not IgE-bearing epidermal Langerhans cells are specific to skin lesions of atopic dermatitis (AD), we performed immunohistochemical and immunoelectron microscopic examinations of dinitrochlorobenzene (DNCB) contact dermatitis lesions provoked in uninvolved skin of eight patients with AD. In all of the eight examined, IgE-positive epidermal Langerhans cells were observed in the DNCB dermatitis lesions. Typical staining of anti-IgE was absent in the epidermis of normal-appearing skin of five patients with AD. Thus, it is likely that IgE positive epidermal Langerhans cells non-specifically occur in different eczematous diseases provoked in patients with AD.
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Barker JNWN, Alegre VA, MacDonald DM (1988) Surface-bound immunoglobulin E on antigen presenting cells in cutaneous tissue of atopic dermatitis. J Invest Dermatol 90:117–121
Bieber T, Dannenberg B, Prinz JC, Rieber P, Stolz W, Braun-Falco O, Ring J (1989) Occurrence of IgE-bearing epidermal Langerhans cells in atopic eczema: a study of the time course of the lesions and with regard to the IgE serum level. J Invest Dermatol 92:215–219
Bruynzeel-Koomen C (1986) IgE on Langerhans cells: new insights into the pathogenesis of atopic dermatitis. Dermatologica 172:181–183
Bruynzeel-Koomen C, Wichen DF, Toonstra J, Berrens L, Bruynzeel PLB (1986) The presence of IgE molecules on epidermal Langerhans cells in patients with atopic dermatitis. Arch Dermatol Res 278:199–205
Hanifin JM, Rajka G (1980) Diagnostic features of atopic dermatitis. Acta Derm Venereol (Stockh) 92[Suppl]:44–47
Holden CA, Morgan EW, MacDonald DM (1982) A technique for immunoultrastructural identification of T6-positive Langerhans cells and indeterminate cells in mycosis fungoides. J Invest Dermatol 79:382–384
Leung DYM, Schneeberger EE, Siraganian RP, Geha RS, Bhan AK (1987) The presence of IgE macrophages and dendritic cells infiltrating into the skin of atopic dermatitis. Clin Immunol Immunopathol 42:328–337
Lever R, Turbitt M, Sanderson A, MacKie R (1987) Immunophenotyping of the cutaneous infiltrate and of the mononuclear cells in the peripheral blood in patients with atopic dermatitis. J Invest Dermatol 89:4–7
McLean IAW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Mitchell EB, Chapman MD, Pope FM, Crow J, Jouhal SS, Platts-Mills TAE (1982) Basophils in allergen-induced patch test sites in atopic dermatitis. Lancet I:127–130
Platts-Mills TAE, Mitchell EB, Rowntree S, Chapman MD, Wilkins SR (1983) The role of dust mite allergens in atopic dermatitis. Clin Exp Dermatol 8:233–247
Reitamo S, Visa K, KÄhönen K, KÄyhkö K, Stubb S, Salo OP (1986) Eczematous reactions in atopic patients caused by epicutaneous testing with inhalant allergens. Br J Dermatol 114:303–309
Ring J, Kunz B, Bieber T, Vieluf D, Przybilla B (1989) The “atopic patch test” with aeroallergens in atopic eczema. J Invest Dermatol 92:507
Schnyder UW, Klunker W (1957) über das phÄnotypische, familienpathologische Verhalten der Atopien. Hautarzt 8:510–511
Taylor CR, Hofman FM, Modlin RL, Rea TH (1983) Immunoperoxidase techniques applied to dermatopathology. J Cutan Pathol 10:145–163
Uehara M (1986) Heterogeneity of serum IgE levels in atopic dermatitis. Acta Derm Venereol (Stockh) 66:404–408
Uehara M, Sawai T (1989) A longitudinal study of contact sensitivity in patients with atopic dermatitis. Arch Dermatol 125:366–368
Uehara M, Sawai T (1989) Familial background of respiratory atopy. A factor of type I allergy to house dust mite in patients with atopic dermatitis. Arch Dermatol 125:939–943
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Sugiura, H., Uehara, M. & Maeda, T. IgE-positive epidermal Langerhans cells in allergic contact dermatitis lesions provoked in patients with atopic dermatitis. Arch Dermatol Res 282, 295–299 (1990). https://doi.org/10.1007/BF00375722
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DOI: https://doi.org/10.1007/BF00375722