Summary
The lens, iris, and ciliary body from the eyes of patients with glaucoma with cataract were examined via electron microscopy, and the following results obtained.
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1.
Zonular bundles were encrusted with accumulations of pseudoexfoliative (PE) materials and fragmented zonular fibrils, especially on their lateral aspects.
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2.
PE materials ranged from 300 to 400 Å in width, consisting of several subunits about 100 Å wide.
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3.
Pigment epithelial cells of the iris and nonpigmented epithelial cells of the ciliary process occasionally contained many phagosomes engulfing pigment granules.
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4.
Near the ciliary process, degenerating zonular bundles were mostly replaced with PE materials and fragmented zonular fibrils, and a gradual transition was observed between them.
From the above findings, it is probable that PE material might be mainly composed of degenerated zonular fibrils, which are presumed to be digested with lysosomal enzymes.
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Arnesen, K., Sunde, O.A., Schultz-Haudt, S.D.: Histochemical studies of the deposits of Busacca in eyes with glaucoma simplex and so-called senile exfoliation of the anterior lens capsule. Acta Ophthalmol. (Kbh.) 41, 80–84 (1963)
Ashton, N., Shakib, M., Collyer, R., Blach, R.: Electron microscopic study of pseudoexfoliation of the lens capsule. I. Lens capsule and zonular fibers. Invest. Ophthalmol. 4, 141–153 (1965)
Bartholomew, R.A.: Lens displacement associated with pseudo-capsular exfoliation — a report on 19 cases in the Southern Bantu. Br. J. Ophthalmol. 54, 744–750 (1970)
Bertelsen, T.I.: Fibrillopathia epithelio-capsularis. The so-called senile exfoliation or pseudo-exfoliation of the anterior lens capsule. Acta Ophthalmol. (Kbh.) 44, 737–750 (1966)
Bertelsen, T.I., Drablos, P.A., Flood, P.R.: The so-called senile exfoliation (pseudo-exfoliation) of the anterior lens capsule, a product of the lens epithelium. Fibrillopathia epithelio-capsularis. Acta Ophthalmol. (Kbh.) 42, 1096–1113 (1964)
Bertelsen, T.I., Ehlers, N.: Morphological and histochemical studies on fibrillopathia epithelio-capsularis. Acta Ophthalmol. (Kbh.) 47, 476–488 (1969)
Blackstad, T.W., Sunde, O.A., Traetteberg, J.: On the ultrastructure of the deposits of Busacca in eyes with glaucoma simplex and so-called senile exfoliation of the anterior lens capsule. Acta Ophthalmol. (Kbh.) 38, 587–598 (1960)
Buddecke, E., Wollensak, J.: Zur Biochemie der Zonulafaser des Rinderauges. Z. Naturforsch. (c) 21B, 337–341 (1966)
Dark, A.J., Streeten, B.W., Cornwall, C.C.: Pseudo-exfoliative disease of the lens: a study in electron microscopy and histochemistry. Br. J. Ophthalmol. 61, 462–472 (1977)
Davanger, M., Pedersen, O.O.: Pseudo-exfoliation material on the anterior lens surface — demonstration and examination of an interfibrillar ground substance. Acta Ophthalmol. (Kbh.) 53, 3–18 (1975)
Dvorak-Theobald, G.: Pseudo-exfoliation of the lens capsule — relation to “true” exfoliation of the lens capsule as reported in the literature and role in the production of glaucoma capsulocuticulare. Am. J. Ophthalmol. 37, 1–12 (1954)
Ghosh, M., Speakman, J.S.: Inclusions in human lens capsule and their relationship to senile exfoliation. Can. J. Ophthalmol. 7, 413–427 (1972)
Ghosh, M., Speakman, J.S.: The ciliary body in senile exfoliation of the lens. Can. J. Ophthalmol. 8, 394–403 (1973)
Ghosh, M., Speakman, J.S.: The iris in senile exfoliation of the lens. Can. J. Ophthalmol. 9, 289–297 (1974)
Glenner, G.G., Kreiser, H.R., Bladen, H.A., Cuatrecasas, P., Eanes, E.D., Ram, J.S., Kanfer, J.N., Delellis, R.A.: Amyloid. VI. A comparison of two morphologic components of human amyloid deposits. J. Histochem. Cytochem. 16, 633–644 (1968)
Hørven, I.: Exfoliation syndrome — a histological and histochemical study. Acta Ophthalmol. (Kbh.) 44, 790–800 (1966)
Raviola, G.: The fine structure of the zonule and ciliary epithelium. Invest. Ophthalmol. 10, 851–869 (1971)
Ringvold, A.: A preliminary report on the amino acid composition of the pseudoexfoliation material (PE material). Exp. Eye Res. 15, 37–42 (1973)
Ringvold, A., Husby, G.: Pseudo-exfoliation material — an amyloid-like substance. Exp. Eye. Res. 17, 289–299 (1973)
Ross, R., Bornstein, P.: The elastic fiber. I. The separation and partial characterization of its macromolecular components. J. Cell Biol. 40, 366–381 (1969)
Shakib, M., Ashton, N., Blach, R.: Electron microscopic study of pseudo-exfoliation of the lens capsule. II. Iris and ciliary body. Invest. Ophthalmol. 4, 154–161 (1965)
Takei, Y., Ozanics, V.: Electron microscopic studies on the zonule. I. Fine structure of normal and ruthenium red-stained zonular fibrils in the rabbit and monkey. Jpn. J. Ophthalmol. 19, 69–93 (1975)
Takei, Y., Smelser, G.K.: Electron microscopic studies on zonular fibers. II. Changes of the zonular fibers after the treatment with collagenase, α-chymotrypsin and hyaluronidase. Albrecht v. Graefes Arch. Klin. Exp. Ophthalmol. 194, 153–173 (1975)
Wollensak, J.: Zonula Zinnii. Fortschr. Augenheilkd. 16, 240–335 (1965)
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Takei, Y., Mizuno, K. Electron-Microscopic study of pseudo- exfoliation of the lens capsule. Albrecht von Graefes Arch. Klin. Ophthalmol. 205, 213–220 (1978). https://doi.org/10.1007/BF00413449
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DOI: https://doi.org/10.1007/BF00413449